Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy
Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy
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DOI：
10.1016/j.fertnstert.2015.01.041
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发表时间：
2015-05-01
影响因子：
6.7
通讯作者：
Masuzaki, Hideaki
中科院分区：
文献类型：
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作者：
Miura, Kiyonori;Higashijima, Ai;Masuzaki, Hideaki
目的：探讨无细胞妊娠相关microrna作为异位妊娠诊断的分子标志物。设计：使用人体血浆样本进行实验室研究。环境：大学医院的研究单位。患者：18例异位妊娠（EP组），12例自然流产（SA组），26例正常单胎妊娠（NP组）。干预：从1.2 mL血浆中提取含有小RNA分子的总RNA。主要观察指标：实时定量逆转录酶聚合酶链反应测定血浆无细胞microrna浓度。结果：证实妊娠相关的无细胞microrna （miR-323-3p、miR-515-3p、miR-517a、miR-517c、miR-518b）血浆浓度和人绒毛膜促性腺激素（hCG）血清浓度在EP、SA、NP患者的血浆或血清浓度有统计学差异。三组患者血浆中无细胞miR-21浓度差异无统计学意义。通过相关系数分析，血清hCG水平与无浆细胞miR-517c、miR-515-3p、miR-517a、miR-518b、miR-323-3p、miR-21水平无相关性。血浆无细胞miR-517a浓度可以区分EP/SA和NP，曲线下面积为0.9654（95%可信区间0.9172-1.0）。血浆无细胞miR-323-3p浓度可以区分EP和SA，曲线下面积为0.7454（95%可信区间为0.5558-0.9349）。结论：无细胞妊娠相关microrna有可能作为异位妊娠的分子标记物。(C) 2015年美国生殖医学学会。
Objective: To investigate cell-free pregnancy-associated microRNAs as molecular markers for the diagnosis of ectopic pregnancy.Design: Laboratory study using human plasma samples.Setting: Research unit in a university hospital.Patient(s): Plasma samples from 18 women with ectopic pregnancies (EP group), 12 women with spontaneous abortion (SA group), and 26 normal women with singleton pregnancies (NP group).Intervention(s): Total RNAs containing small RNA molecules extracted from 1.2 mL of plasma.Main Outcome Measure(s): Plasma concentrations of cell-free microRNAs measured by quantitative real-time reverse-transcriptase polymerase chain reaction.Result(s): Plasma concentrations of cell-free pregnancy-associated microRNAs (miR-323-3p, miR-515-3p, miR-517a, miR-517c, and miR-518b) and serum concentration of human chorionic gonadotropin (hCG) were confirmed to have statistically significantly different plasma or serum concentrations in women with EP, SA, or NP. There was no statistically significant difference in the plasma concentrations of cell-free miR-21 between the three groups. By correlation coefficient analysis, no relationship was detected between serum hCG levels and plasma cell-free miR-517c, miR-515-3p, miR-517a, miR-518b, miR-323-3p, or miR-21 levels. Plasma concentrations of cell-free miR-517a could distinguish EP/SA from NP, yielding an area under the curve of 0.9654 (95% confidence interval, 0.9172-1.0). Plasma concentrations of cell-free miR-323-3p could distinguish EP from SA, yielding an area under the curve of 0.7454 (95% confidence interval, 0.5558-0.9349).Conclusion(s): Cell-free pregnancy-associated microRNAs have potential as molecular markers of ectopic pregnancy. (C) 2015 by American Society for Reproductive Medicine.