Construction of Flower-specific Chimeric Promoters and Analysis of Their Activities in Transgenic Torenia
Construction of Flower-specific Chimeric Promoters and Analysis of Their Activities in Transgenic Torenia
复制标题
转基因蓝猪草花特异性嵌合启动子的构建及其活性分析
DOI：
10.1007/s11105-013-0646-4
复制
发表时间：
2014-02-01
影响因子：
2.1
通讯作者：
Wang, Yuejin
中科院分区：
文献类型：
--
作者：
Du, Lingjuan;Lou, Qian;Wang, Yuejin
花特异性启动子能够转基因增强有价值的观赏性状，包括花的形状和颜色。然而，强的，组织特异性启动子的鉴定仍然是一个限制因素。为了获得增强的花特异性启动子，我们构建了4个嵌合启动子（p35 S-PCHS-Ω、p35 S-LCHS-Ω、pOCS-PCHS-Ω和pOCS-LCHS-Ω），它们将35 S或OCS增强子与来自矮牵牛的302 bp CHSA核心启动子片段（PCHS）或来自百合的307 bp CHS核心启动子片段（LCHS）融合，并且还含有Ω元件（Ω）。每个启动子融合到β-葡萄糖醛酸酶（GUS）报告基因，我们检查了转基因蓝猪耳中GUS表达的水平和组织特异性。p35 S-PCHS-Ω和p35 S-LCHS-Ω在所有组织中驱动强的组成型GUS表达，特别是在有色花冠（p35 S-PCHS-Ω）或有色花冠和根（p35 S-LCHS-Ω）中。pOCS-PCHS-Ω在有色花冠中的GUS表达量高于其它组织，但表达量低于p35 S-PCHS-Ω。pOCS-LCHS-Ω不仅在有色花冠中而且在根中驱动GUS。
Flower-specific promoters can enable transgenic enhancement of valuable ornamental traits, including flower shape and color. However, the identification of strong, tissue-specific promoters remains a limiting factor. To obtain enhanced flower-specific promoters, we constructed four chimeric promoters (p35S-PCHS-Ω, p35S-LCHS-Ω, pOCS-PCHS-Ω and pOCS-LCHS-Ω) combining the 35S or OCS enhancer fused to a 302 bp CHSA core promoter fragment from petunia (PCHS) or a 307 bp CHS core promoter fragment from lily (LCHS), and also containing an omega element (Ω). Each promoter was fused to the β-glucuronidase (GUS) reporter gene, and we examined the levels and tissue specificity of GUS expression in transgenic Torenia fournieri. p35S-PCHS-Ω and p35S-LCHS-Ω drove strong, constitutive GUS expression in all tissues, especially in colored corollas (p35S-PCHS-Ω) or in colored corollas and roots (p35S-LCHS-Ω). pOCS-PCHS-Ω drove stronger GUS expression in colored corollas than in other tissues but expression was weaker than that of p35S-PCHS-Ω. pOCS-LCHS-Ω drove GUS in colored corollas but also in roots.