Pharmacological testing in the laser-induced monkey glaucoma model.
Pharmacological testing in the laser-induced monkey glaucoma model.
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DOI：
10.3109/02713688509020033
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发表时间：
1985
影响因子：
2
通讯作者：
P. Lee;S. Podos;J. Howard-Williams;C. Severin;A. D. Rose;M. Siegel
中科院分区：
文献类型：
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作者：
P. Lee;S. Podos;J. Howard-Williams;C. Severin;A. D. Rose;M. Siegel
用氩激光照射猕猴小梁网诱发青光眼。重复治疗往往是必要的，广泛的眼内压波动的特点。基线眼内压用经校准的呼吸压力计每小时测量一次，持续6小时。在随后的一天，进行基线测量，施加50微升待测试的药物，并且每6小时重复测量眼内压。在至少8只眼睛中测试了噻吗洛尔、肾上腺素、毛果芸香碱、钒酸盐、前列腺素F2 α（PGF2 α）、毛喉素和柯那生对眼内压的影响。0.5%噻吗洛尔、2%肾上腺素、4%毛果芸香碱、1%钒酸盐、500 μ g PGF 2 α和1%毛喉素可显著降低眼内压（p <0.05）。5%柯楠碱没有显著降低眼内压。眼压描记术显示4%匹罗卡品给药后2小时，眼内压降低，与流出功能增加相关。该青光眼动物模型可用于研究通过多种机制降低眼内压的药物。
Glaucoma was induced in cynomolgus monkeys by photocoagulating the trabecular meshwork with the argon laser. Repeat treatments were often necessary and wide intraocular pressure fluctuations were characteristic. Baseline intraocular pressure was measured with a calibrated pneumatonometer hourly for six hours. On a succeeding day a baseline measurement was made, 50 microliter of the drug to be tested applied, and six hourly measurements of intraocular pressure repeated. The effects on intraocular pressure of timolol, epinephrine, pilocarpine, vanadate, prostaglandin F2 alpha (PGF2 alpha), forskolin, and corynanthine were tested in at least eight eyes. Significant (p less than 0.05) reductions of intraocular pressure were produced by 0.5% timolol, 2% epinephrine, 4% pilocarpine, 1% vanadate, 500 micrograms of PGF2 alpha and 1% forskolin. Five per cent corynanthine produced no significant lowering of intraocular pressure. Tonography revealed an increased outflow facility associated with the reduction of intraocular pressure 2 hours after the administration of 4% pilocarpine. This glaucoma animal model may be useful in investigating agents that lower intraocular pressure by a variety of mechanisms.