Prophylactic Supplementation of Bifidobacterium longum 5(1A) Protects Mice from Ovariectomy-Induced Exacerbated Allergic Airway Inflammation and Airway Hyperresponsiveness.
Prophylactic Supplementation of Bifidobacterium longum 5(1A) Protects Mice from Ovariectomy-Induced Exacerbated Allergic Airway Inflammation and Airway Hyperresponsiveness.
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DOI：
10.3389/fmicb.2017.01732
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发表时间：
2017
影响因子：
5.2
通讯作者：
Ferreira CM
中科院分区：
文献类型：
--
作者：
Mendes E;Acetturi BG;Thomas AM;Martins FDS;Crisma AR;Murata G;Braga TT;Camâra NOS;Franco ALDS;Setubal JC;Ribeiro WR;Valduga CJ;Curi R;Dias-Neto E;Tavares-de-Lima W;Ferreira CM
哮喘是一种慢性炎症性疾病，青春期后女性发病率高于男性，女性的哮喘症状和严重程度会在月经和更年期发生变化。最近，有证据表明，微生物群、女性性激素和免疫力之间的相互作用与自身免疫性疾病的发生有关。然而，没有研究调查治疗性肠道微生物群调节策略是否会影响月经和更年期期间的哮喘恶化。在这里，我们的目的是检查益生菌长双歧杆菌 51A 对过敏性卵巢切除小鼠气道炎症恶化的预防作用。我们首先评估了卵巢切除术后 10 天小鼠的肠道微生物群组成和多样性。接下来，我们检查了切除卵巢的过敏小鼠再次暴露于抗原（卵清蛋白）是否会导致肺部炎症加剧。最后，我们评估了长双歧杆菌51A对肺部炎症和气道高反应性的预防和治疗效果。我们的结果表明，虽然卵巢切除术没有引起该动物模型肠道微生物群组成和多样性的改变，但在卵巢切除术10天后，预防性使用长双歧杆菌51A（而不是手术后使用）能够减轻卵巢切除过敏小鼠加重的肺部炎症和高反应性。 B. longum 51A 的这种预防作用涉及乙酸盐的产生，这会导致粪便乙酸盐水平增加，从而导致卵巢切除过敏小鼠的 Treg 细胞增加。
Asthma is a chronic inflammatory disease that affects more females than males after puberty, and its symptoms and severity in women change during menstruation and menopause. Recently, evidence has demonstrated that interactions among the microbiota, female sex hormones, and immunity are associated with the development of autoimmune diseases. However, no studies have investigated if therapeutic gut microbiota modulation strategies could affect asthma exacerbation during menstruation and menopause. Here we aimed to examine the preventive effects of a probiotic, Bifidobacterium longum 51A, on airway inflammation exacerbation in allergic ovariectomized mice. We first evaluated the gut microbiota composition and diversity in mice 10 days after ovariectomy. Next, we examined whether re-exposure of ovariectomized allergic mice to antigen (ovalbumin) would lead to exacerbation of lung inflammation. Finally, we evaluated the preventive and treatment effect of B. longum 51A on lung inflammation and airway hyperresponsiveness. Our results showed that whereas ovariectomy caused no alterations in the gut microbiota composition and diversity in this animal model, 10 days after ovariectomy, preventive use administration of B. longum 51A, rather than its use after surgery was capable of attenuate the exacerbated lung inflammation and hyperresponsiveness in ovariectomized allergic mice. This prophylactic effect of B. longum 51A involves acetate production, which led to increased fecal acetate levels and, consequently, increased Treg cells in ovariectomized allergic mice.