Attachment of cells to basement membrane collagen type IV.
Attachment of cells to basement membrane collagen type IV.
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细胞与 IV 型胶原基底膜的附着。
DOI：
10.1083/jcb.103.4.1569
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发表时间：
1986-10
期刊：
影响因子：
--
通讯作者：
Timpl R
中科院分区：
文献类型：
--
作者：
Aumailley M;Timpl R
十个不同的细胞系检查，三个显示出不同的附着和胶原IV基板上的蔓延，既不附着也不蔓延，通过添加可溶性层粘连蛋白或纤连蛋白增强。放线菌酮或层粘连蛋白抗体不抑制该反应，表明直接附着于IV型胶原而不需要介体蛋白。细胞结合位点被定位于IV型胶原的主要三螺旋结构域，并且需要完整的三螺旋构象来进行活性。纤连蛋白表现出优先结合到变性胶原蛋白IV必要的介导细胞结合到基板。胶原IV的纤连蛋白结合位点被映射到主要三螺旋结构域的未折叠结构，并显示出与胶原I的纤连蛋白结合位点相似的特异性。这些数据扩展了先前对位于基底膜胶原IV三螺旋中的生物学潜在结合位点的观察。
Of ten different cell lines examined, three showed distinct attachment and spreading on collagen IV substrates, and neither attachment nor spreading was enhanced by adding soluble laminin or fibronectin. This reaction was not inhibited by cycloheximide or antibodies to laminin, indicating a direct attachment to collagen IV without the need of mediator proteins. Cell-binding sites were localized to the major triple-helical domain of collagen IV and required an intact triple helical conformation for activity. Fibronectin showed preferential binding to denatured collagen IV necessary to mediate cell binding to the substrate. Fibronectin binding sites of collagen IV were mapped to unfolded structures of the major triple-helical domain and show a similar specificity to fibronectin-binding sites of collagen I. The data extend previous observations on biologically potential binding sites located in the triple helix of basement membrane collagen IV.