Effect of buformin and metformin on formation of advanced glycation end products by methylglyoxal
Effect of buformin and metformin on formation of advanced glycation end products by methylglyoxal
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DOI：
10.1016/j.cccn.2005.02.012
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发表时间：
2005-08-01
影响因子：
5
通讯作者：
Hirano, K
中科院分区：
文献类型：
--
作者：
Kiho, T;Kato, M;Hirano, K
背景：晚期糖基化终末产物(AGE)在各种组织中的形成和积聚被认为与长期糖尿病的衰老过程和并发症有关。首次研究了氨基胍作为糖基化终末产物的抑制剂，并报道了双胍类化合物与甲基乙醛等反应性二羰基前体的反应。方法：采用特异性荧光分析、酶联免疫吸附试验和抗AGE抗体的Western印迹分析等方法，研究了丁双胍和二甲双胍对糖基化终末产物形成的影响。结果：丁双胍是一种比二甲双胍更有效的糖基化终末产物形成抑制剂，提示丁双胍的氨基通过捕获甲基乙醛的羰基来抑制AGE的形成。结论：除二甲双胍外，丁双胍还可用于糖尿病并发症的预防。(C)2005 Elsevier B.V.保留所有权利。
Background: The formation and accumulation of advanced glycation end products (AGE) in various tissues are known to be involved in the aging process and complications of long-term diabetes. Aminoguanidine as AGE inhibitors was first studied, and metformin as biguanide compounds have been reported to react with reactive dicarbonyl precursors such as methylglyoxal.Methods: We studied the effects of the biguanides of buformin and metformin on AGE formation by the methods of specific fluorescence, and enzyme-linked immunosorbent assay and a Western blot analysis using the anti-AGE antibody after incubating BSA or RNase with methylglyoxal.Results: Buformin is a more potent inhibitor of AGE formation than metformin, and suggests that the amino group of buformin trap the carbonyl group of methylglyoxal to suppress fort-nation of AGE.Conclusion: In addition to that of metformin, buformin may be clinically useful to prevent diabetic complications. (c) 2005 Elsevier B.V. All rights reserved.