Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa β and toll-like receptor pathways
Ocean acidification weakens the immune response of blood clam through hampering the NF-kappa β and toll-like receptor pathways
复制标题
海洋酸化通过阻碍 NF-κβ 和 Toll 样受体途径削弱血蛤的免疫反应
DOI：
10.1016/j.fsi.2016.04.030
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发表时间：
2016-07-01
影响因子：
4.7
通讯作者：
Liu, Guangxu
中科院分区：
文献类型：
--
作者：
Liu, Saixi;Shi, Wei;Liu, Guangxu
pCO(2)驱动的海洋酸化对海洋双壳类动物免疫的影响仍然知之甚少。迄今为止，这种影响只在少数双壳类物种中进行了研究，其潜在的分子机制仍然未知。在本研究中，研究了现实未来海洋pCO(2)水平（pH分别为8.1、7.8和7.4）对典型底栖双壳类动物血蛤（Tegillarca granosa）的血细胞总数、吞噬状态、血细胞类型组成以及NF-kappa β信号通路和toll样受体通路中12个基因表达水平的影响。结果显示，暴露在高pCO环境下，THC数量和吞噬频率均显著降低，红细胞和嗜碱性粒细胞百分比分别显著减少和增加(2)。此外，暴露于pCO(2)酸化的海水中，一般会导致NF-kappa β信号通路和toll样受体通路的诱导剂和关键应答基因显著下调。本研究的结果表明，海洋酸化可能会阻碍双壳动物的免疫反应，从而使个体更容易受到病毒和细菌等病原体的攻击。(C) 2016 Elsevier Ltd.版权所有。
The impact of pCO(2) driven ocean acidification on marine bivalve immunity remains poorly understood. To date, this impact has only been investigated in a few bivalve species and the underlying molecular mechanism remains unknown. In the present study, the effects of the realistic future ocean pCO(2) levels (pH at 8.1, 7.8, and 7.4) on the total number of haemocyte cells (THC), phagocytosis status, blood cell types composition, and expression levels of twelve genes from the NF-kappa beta signaling and toll-like receptor pathways of a typical bottom burrowing bivalve, blood clam (Tegillarca granosa), were investigated. The results obtained showed that while both THC number and phagocytosis frequency were significantly reduced, the percentage of red and basophil granulocytes were significantly decreased and increased, respectively, upon exposure to elevated pCO(2). In addition, exposure to pCO(2) acidified seawater generally led to a significant down-regulation in the inducer and key response genes of NF-kappa beta signaling and toll -like receptor pathways. The results of the present study revealed that ocean acidification may hamper immune responses of the bivalve T granosa which subsequently render individuals more susceptible to pathogens attacks such as those from virus and bacteria. (C) 2016 Elsevier Ltd. All rights reserved.