ULTRASOUND BACKSCATTER MICROSCOPE ANALYSIS OF EARLY MOUSE EMBRYONIC BRAIN-DEVELOPMENT
ULTRASOUND BACKSCATTER MICROSCOPE ANALYSIS OF EARLY MOUSE EMBRYONIC BRAIN-DEVELOPMENT
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DOI：
10.1073/pnas.92.6.2239
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发表时间：
1995-03-14
影响因子：
11.1
通讯作者：
JOYNER, AL
中科院分区：
文献类型：
--
作者：
TURNBULL, DH;BLOOMFIELD, TS;JOYNER, AL
小鼠发育和遗传分析的历史使其成为研究哺乳动物胚胎发生的首选模型。目前缺乏一种简单的技术，有效地分析早期小鼠突变体表型在子宫内。我们展示了应用实时成像方法称为超声背向散射显微镜可视化小鼠早期胚胎神经管和心脏。该方法用于研究胚胎发生9.5至11.5天的子宫内活胚胎，空间分辨率接近50 μ m。培养胚胎的超声背向散射显微镜图像使心脏腔室可视化成为可能。这种非侵入性成像方法也被用于分析神经管缺陷。与Wnt-1原癌基因无效突变相关的中后脑缺失在10.5天和11.5天胚胎的超声背散射显微镜图像上很容易识别。计算机生成的体积渲染的神经管腔从三维图像数据。这使得Wnt-1突变体表型的定义更加清晰。这些成像技术在研究小鼠在子宫内的发育过程中应该有相当大的用处。
The history of developmental and genetic analysis in the mouse has made it the model of choice for studying mammalian embryogenesis. Presently lacking is a simple technique for efficiently analyzing early mouse mutant phenotypes in utero. We demonstrate application of a real-time imaging method called ultrasound backscatter microscopy for visualizing mouse early embryonic neural tubes and hearts. This method was used to study live embryos in utero between 9.5 and 11.5 days of embryogenesis, with a spatial resolution close to 50 mu m. Ultrasound backscatter microscope images of cultured embryos made it possible to visualize the heart chambers. This noninvasive imaging method was also used for analyzing a neural tube defect. The midhindbrain deletion associated with a null mutation of the Wnt-1 protooncogene was easily recognizable on ultrasound backscatter microscope images of 10.5- and 11.5-day embryos. Computer-generated volumetric renderings of the neural tube cavities were made from three-dimensional image data. This allowed a much clearer definition of the Wnt-1 mutant phenotype. These imaging techniques should be of considerable use in studying mouse development in utero.