AN EXPERIMENTAL VACCINE COCKTAIL FOR PLASMODIUM-FALCIPARUM MALARIA
AN EXPERIMENTAL VACCINE COCKTAIL FOR PLASMODIUM-FALCIPARUM MALARIA
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DOI：
10.1016/0264-410x(93)90287-8
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发表时间：
1993-03-01
期刊：
影响因子：
5.5
通讯作者：
BARR, PJ
中科院分区：
文献类型：
--
作者：
BATHURST, IC;GIBSON, HL;BARR, PJ
恶性疟原虫几个不同生命周期阶段的表面蛋白在酵母酿酒酵母中高水平表达。纯化的蛋白质，无论是单独的和在鸡尾酒中，用于免疫小鼠和山羊与弗氏佐剂或基于胞壁酰三肽的佐剂。通过酶联免疫吸附试验和抗血清抑制（1）活子孢子对肝细胞的侵入，（2）活裂殖子对人红细胞的体外侵入，和（3）蚊子载体中卵母细胞的发育的能力来测量免疫应答。这些结果表明，不同阶段特异性抗原的混合物可以提供在其生命周期的多个阶段阻断疟原虫发育所必需的成分。
Surface proteins from several different life-cycle stages of the malaria parasite Plasmodium falciparum were expressed at high levels in the yeast Saccharomyces cerevisiae. Purified proteins, both individually and in cocktails, were used to immunize mice and goats in conjunction with either Freund's adjuvant or a muramyl tripeptide-based adjuvant. Immune responses were measured by enzyme-linked immunosorbent assays and by the ability of antisera to inhibit (1) the invasion of hepatocytes by live sporozoites, (2) in vitro invasion of human erythrocytes by live merozoites, and (3) the development of oocytes in the mosquito vector. These results suggest that cocktails of different stage-specific antigens can provide the components necessary to block the development of the malaria parasite at multiple stages of its life cycle.