REGULATION OF WATER-FLOW BY ACTIN-BINDING PROTEIN-INDUCED ACTIN GELATION
REGULATION OF WATER-FLOW BY ACTIN-BINDING PROTEIN-INDUCED ACTIN GELATION
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DOI：
10.1016/s0006-3495(92)81938-0
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发表时间：
1992-05-01
影响因子：
3.4
通讯作者：
STOSSEL, TP
中科院分区：
文献类型：
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作者：
ITO, T;SUZUKI, A;STOSSEL, TP
肌动蛋白丝抑制渗透驱动的水流（Ito, T., K.S. Zaner, and T.P. Stossel. 1987. Biophys. J.51:745-753）。在这里，我们表明肌动蛋白凝胶蛋白，肌动蛋白结合蛋白（ABP），阻碍肌动蛋白丝溶液的渗透收缩和膨胀，并显着降低这种抑制所需的肌动蛋白丝的浓度。这些效应取决于肌动蛋白丝固定，因为引起肌动蛋白丝水流初始损害的 ABP 浓度对应于粘度测量的凝胶点，并且因为非共价切断肌动蛋白丝的凝溶胶蛋白，使肌动蛋白凝胶溶胶并恢复由 ABP 交联的肌动蛋白溶液中的水流。由于 ABP 在许多真核细胞周围凝胶化肌动蛋白丝，因此这种肌动蛋白网络可能有助于生理细胞体积调节。
Actin filaments inhibit osmotically driven water flow (Ito, T., K.S. Zaner, and T.P. Stossel. 1987. Biophys. J.51:745-753). Here we show that the actin gelation protein, actin-binding protein (ABP), impedes both osmotic shrinkage and swelling of an actin filament solution and reduces markedly the concentration of actin filaments required for this inhibition. These effects depend on actin filament immobilization, because the ABP concentration that causes initial impairment of water flow by actin filaments corresponds to the gel point measured viscometrically and because gelsolin, which noncovalently severs actin filaments, solates actin gels and restores water flow in a solution of actin cross-linked by ABP. Since ABP gels actin filaments in the periphery of many eukaryotic cells, such actin networks may contribute to physiological cell volume regulation.