REGULATORY INTERACTIONS BETWEEN MITOCHONDRIAL GENES - INTERACTIONS BETWEEN 2 MOSAIC GENES
REGULATORY INTERACTIONS BETWEEN MITOCHONDRIAL GENES - INTERACTIONS BETWEEN 2 MOSAIC GENES
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DOI：
10.1073/pnas.78.3.1778
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MAHLER, HR
中科院分区：
文献类型：
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作者：
DHAWALE, S;HANSON, DK;MAHLER, HR
研究了含有特定插入序列的2个镶嵌基因cob（细胞色素b基因）和ox3（细胞色素氧化酶I亚基基因）的酿酒酵母菌株的线粒体DNA和表型。研究结果表明：包含内含子box7的下游干预序列的存在足以通过cob调节ox3 （BOX表型）；cob上游的2个序列（含内含子位点box3和box10）和ox3中的2个序列是可省去的。没有上游2个序列的菌株仍然包含下游序列和指定功能性反作用元件的能力。该片段的突变病变在表型上与box7突变难以区分，包括具有同源氨基酸序列的多肽的积累。分解代谢物敏感的BOX表型，突变体在第1外显子的特征，需要同时存在相邻的干预序列。提出了一个模型，其中cob基因的内含子（位点box7）指定的假设产物控制第二个马赛克基因（ox3）的表达。
The mitochondrial DNA and the phenotypes of strains of Saccharomyces cerevisiae with specific intervening sequences in 2 mosaic genes, cob (the gene for apocytochrome b) and oxi3 (the gene for subunit I of cytochrome oxidase), were studied. The results suggest the following conclusions. The presence of an intervening sequence downstream encompassing the intron box7 is sufficient for the regulation of oxi3 by cob (BOX phenotype); 2 sequences (containing intron loci box3 and box10) upstream in cob and 2 in oxi3 are dispensable. Strains without the 2 sequences upstream still contain the dowstream sequence and the competence to specify a functional trans-acting element. Mutational lesions in this segment are phenotypically indistinguishable from box7 mutants, including the accumulation of polypeptides with homologous amino acid sequences. A catabolite-sensitive BOX phenotype, characteristic of mutants in the 1st exon, requires the simultaneous presence of an adjacent intervening sequence. A model is presented in which a hypothetical product specified by an intron (locus box7) of the cob gene controls the expression of a 2nd mosaic gene (oxi3).