Chlorogenic acid attenuates lipopolysaccharide-induced mice mastitis by suppressing TLR4-mediated NF-κB signaling pathway
Chlorogenic acid attenuates lipopolysaccharide-induced mice mastitis by suppressing TLR4-mediated NF-κB signaling pathway
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DOI：
10.1016/j.ejphar.2014.01.015
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发表时间：
2014-04-15
影响因子：
5
通讯作者：
Zhang Naisheng
中科院分区：
文献类型：
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作者：
Gao Ruifeng;Fu Yunhe;Zhang Naisheng
绿原酸（CGA）是饮食中最丰富的多酚之一，据报道具有有效的抗炎特性。然而，CGA对脂多糖（LPS）诱导的小鼠乳腺炎的影响尚未见研究。本研究的目的是阐明CGA是否可以改善LPS诱导的小鼠乳腺炎的炎症反应，并阐明其可能的机制。通过乳腺导管注射LPS，建立小鼠乳腺炎模型。CGA分别在LPS诱导前1 h和诱导后12 h以125、25、50 mg/kg的剂量腹腔注射。本研究通过组织病理学检查、酶联免疫吸附试验和western blot分析，评价CGA对LPS诱导小鼠乳腺炎的影响。结果显示，与LPS组相比，CGA显著降低了tnf - α、IL-1 β和IL-6的产生。此外，western blot分析显示，CGA可以抑制TLR4的表达以及LPS诱导的NF-kappa B和I -kappa B的磷酸化。这些结果表明，CGA对lps诱导的乳腺炎的抗炎作用可能是由于其抑制tlr4介导的NF-kappa B信号通路的能力。因此，CGA可能是一种有效的治疗试剂，用于预防埃希菌引起的乳腺炎期间遇到的免疫病理。(C) 2014 Elsevier B.V.版权所有
Chlorogenic acid (CGA), one of the most abundant polyphenols in the diet, has been reported to have potent anti-inflammatory properties. However, the effect of CGA on lipopolysaccharide (LPS) induced mice mastitis has not been investigated. The purpose of the present study was to elucidate whether CGA could ameliorate the inflammation response in LPS induced mice mastitis and to clarify the possible mechanism. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. CGA was administered intraperitoneally with the dose of 125, 25, and 50 mg/kg respectively 1 h before and 12 h after induction of LPS. In this study, the effect of CGA on LPS induced mice mastitis was assessed through histopathological examination, ELISA assay, and western blot analysis. The results showed that CGA significantly reduced TNF-alpha, IL-1 beta, and IL-6 production compared with LPS group. Besides, western blot analysis showed that CGA could inhibit the expression of TLR4 and the phosphorylation of NF-kappa B and I kappa B induced by LPS. These results suggested that anti-inflammatory effects of CGA against LPS-induced mastitis may be due to its ability to inhibit TLR4-mediated NF-kappa B signaling pathway. Therefore, CGA may be a potent therapeutic reagent for the prevention of the immunopathology encountered during Escherichia wit elicited mastitis. (C) 2014 Elsevier B.V. All rights resented.