Patterning of endocytic vesicles and its control by voltage-gated Na+ channel activity in rat prostate cancer cells:: fractal analyses
Patterning of endocytic vesicles and its control by voltage-gated Na+ channel activity in rat prostate cancer cells:: fractal analyses
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DOI：
10.1007/s00249-004-0394-3
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发表时间：
2004-10-01
影响因子：
2
通讯作者：
Djamgoz, MBA
中科院分区：
文献类型：
--
作者：
Krasowska, M;Grzywna, ZJ;Djamgoz, MBA
采用分形方法分析了两种大鼠前列腺癌细胞系（Mat-LyLu和AT-2）的分泌膜活性的定量差异的强和弱转移潜力，分别。通过辣根过氧化物酶摄取测定每个细胞的内吞活性。通过多重分形分析：广义分形维数（D-q）及其勒让德变换f（alpha），以及分区迭代函数系统-半分形（PIFS-SF）分析的数字图像的水泡染色模式进行了评价。这些方法一致表明，在控制条件下，所有的多重分形参数和PIFS-SF代码确定的值更大的Mat-LyLu相比，AT-2细胞。这通常与强转移性Mat-LyLu细胞的内吞/囊泡活性比相应的弱转移性AT-2细胞更发达一致。所有研究的参数对河豚毒素（TTX）预处理的细胞，阻断电压门控Na+通道（VGSC）敏感。一些参数对VGSC活性具有“简单”依赖性，由此用TTX预处理降低了MAT-LyLu细胞的值并消除了两种细胞系之间的差异。然而，对于其他参数，有一个“复杂的”依赖VGSC活动。可能的物理/生理意义的数学参数的研究和性质的参与VGSC活动控制的内吞/分泌进行了讨论。
Fractal methods were used to analyze quantitative differences in secretory membrane activities of two rat prostate cancer cell lines (Mat-LyLu and AT-2) of strong and weak metastatic potential, respectively. Each cell's endocytic activity was determined by horseradish peroxidase uptake. Digital images of the patterns of vesicular staining were evaluated by multifractal analyses: generalized fractal dimension (D-q) and its Legendre transform f(alpha), as well as partitioned iterated function system-semifractal (PIFS-SF) analysis. These approaches revealed consistently that, under control conditions, all multifractal parameters and PIFS-SF codes determined had values greater for Mat-LyLu compared with AT-2 cells. This would agree generally with the endocytic/vesicular activity of the strongly metastatic Mat-LyLu cells being more developed than the corresponding weakly metastatic AT-2 cells. All the parameters studied were sensitive to tetrodotoxin (TTX) pre-treatment of the cells, which blocked voltage-gated Na+ channels (VGSCs). Some of the parameters had a "simple" dependence on VGSC activity, whereby pre-treatment with TTX reduced the values for the MAT-LyLu cells and eliminated the differences between the two cell lines. For other parameters, however, there was a "complex" dependence on VGSC activity. The possible physical/physiological meaning of the mathematical parameters studied and the nature of involvement of VGSC activity in control of endocytosis/secretion are discussed.