Protein and mucin retention on oral mucosal surfaces in dry mouth patients
Protein and mucin retention on oral mucosal surfaces in dry mouth patients
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DOI：
10.1111/j.1600-0722.2010.00728.x
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发表时间：
2010-06-01
影响因子：
1.9
通讯作者：
Proctor, Gordon B.
中科院分区：
文献类型：
--
作者：
Pramanik, Rashida;Osailan, Samira M.;Proctor, Gordon B.
口腔动态平衡在很大程度上取决于唾液中存在的蛋白质和粘蛋白，它们覆盖着所有口腔表面。本研究比较了唾液流量正常的受试者和因唾液功能减退引起的口干患者粘膜表面残留液的蛋白质组成。用纸条收集残留的粘膜液样本，然后进行蛋白质电泳和免疫印迹分析。在患者和对照组中，粘膜表面的残留液(对照组中的前舌除外)的蛋白质浓度高于未经刺激的全口唾液。在对照组中，高分子量粘蛋白(MUC5B)在舌前部的含量高于在其他表面的含量。在不能提供可测量唾液样本的口干患者中，MUC5B通常仍然存在于所有粘膜表面，但在舌前部的数量减少。膜结合型粘蛋白MUC1在患者和对照组的颊和唇表面显著。Statherin仍然存在于干燥的表面以去除唾液，这表明它可能以蛋白质膜的形式被吸附。结论：口干患者口腔黏膜表面可以保留MUC5B和其他唾液蛋白，尽管这些蛋白的功能完整性尚不确定。
Oral homeostasis depends largely on proteins and mucins present in saliva that coat all oral surfaces. The present study compared the protein composition of residual fluid on mucosal surfaces in subjects with normal salivary flow with that of patients with dry mouth caused by salivary hypofunction. Samples of residual mucosal fluid were collected using paper strips and then analysed by protein electrophoresis and immunoblotting. In both patients and controls, residual fluids on mucosal surfaces (except the anterior tongue in control subjects) had higher protein concentrations than unstimulated whole-mouth saliva. High-molecular-weight mucin (MUC5B) was present in greater amounts on the anterior tongue than on other surfaces in control subjects. In dry mouth patients who were unable to provide a measurable saliva sample, MUC5B was often still present on all mucosal surfaces but in reduced amounts on the anterior tongue. The membrane-bound mucin, MUC1, was prominent on buccal and labial surfaces in patients and controls. Statherin was still present on surfaces that were dried to remove salivary fluid, suggesting that it may be adsorbed as a protein pellicle. It is concluded that oral mucosal surfaces in dry mouth patients can retain MUC5B and other salivary proteins, although the functional integrity of these proteins is uncertain.