Transmission electron microscopy of early plaque formation on dental materials in vivo
Transmission electron microscopy of early plaque formation on dental materials in vivo
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DOI：
10.1046/j.0909-8836.1999.eos107109.x
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发表时间：
1999-02-01
影响因子：
1.9
通讯作者：
Hannig, M
中科院分区：
文献类型：
--
作者：
Hannig, M
这项体内研究描述了各种牙科材料上早期菌斑形成的超微结构模式。将汞合金、铸造合金、钛、陶瓷、玻璃聚烯烃酸盐粘固剂、复合树脂、未填充树脂和牛牙釉质的试件使用可移动口内sprint附着于3名受试者的上第一磨牙的颊面和舌面。将样本暴露于口腔环境24 h，随后进行透射电子显微镜评价。在不同材料表面形成的早期斑块的超微结构外观中，只能检测到不太明显的变化。然而，电子显微镜观察显示，口腔和舌安装的测试件之间的早期生物膜形成的明显差异。虽然在舌侧位置佩戴的样本的细菌定植仍然限于表面不规则区域中单个微生物的粘附，但在颊侧区域携带的所有样本上观察到微生物的多层粘附。它的结论是，固体表面上的早期菌斑形成的影响主要是由口腔环境，而不是由材料依赖的参数。这些发现可以归因于薄膜层的存在，其显然掩盖了材料之间在表面性质和生物相容性方面的任何差异。
This in vivo study describes the ultrastructural pattern of early plaque formation on various dental materials. Test pieces of amalgam, casting alloys, titanium, ceramics, glass polyalkenoate cement, composite resins, unfilled resins, and bovine enamel were attached to the buccal and lingual surfaces of the upper first molars in 3 subjects using removable intraoral sprints. Specimens were exposed to the oral environment over a period of 24 h and subsequently processed for transmission electron microscopic evaluation. Only less pronounced variations could be detected in the ultrastructural appearance of the early plaque formed on the different material surfaces. However, electron microscopic observations revealed distinct differences in early biofilm formation between buccally and lingually mounted test pieces. While the bacterial colonization of specimens worn in the lingual position remained limited to the adherence of individual micro-organisms in the area of surface irregularities, a multi-layer adherence of micro-organisms was observed on all specimens carried in buccal areas. It is concluded that early plaque formation on solid surfaces is influenced predominantly by the oral environment rather than by material-dependent parameters. These findings may be ascribed to the presence of the pellicle layer, which apparently masks any difference among materials, with regard to surface properties and biocompatibility.