Age-related changes in the expression of Alzheimer's beta APP in the brain of senescence accelerated mouse (SAM)-P/10
Age-related changes in the expression of Alzheimer's beta APP in the brain of senescence accelerated mouse (SAM)-P/10
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DOI：
10.1097/00001756-199705060-00033
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发表时间：
1997-05-06
期刊：
影响因子：
1.7
通讯作者：
Suh, YH
中科院分区：
文献类型：
--
作者：
Jeong, SJ;Kim, K;Suh, YH
阿尔茨海默病（AD）患者表现出记忆丧失和认知缺陷，其分子机制尚不完全清楚。我们使用逆转录聚合酶链反应（RT-PCR）和Western blot检测了衰老加速小鼠（SAM） P/10和抗衰老小鼠（SAM） r /1脑中β -淀粉样蛋白前体蛋白（beta APP）表达水平的年龄相关变化，这表明衰老加速小鼠（SAM） P/10脑萎缩和学习记忆障碍。SAM-P/10大鼠海马β APP mRNA和蛋白水平均随年龄增长而升高，在8月龄时达到峰值。相比之下，SAM-R/1海马区β APP蛋白水平随年龄增长而下降，β APP mRNA水平变化不显著。在大脑皮层、丘脑/中脑和小脑脑干等其他脑区，β APP mRNA和蛋白水平没有随年龄变化。这些结果提示海马β APP过表达可能与SAM-P/10特征性记忆丧失有关。
PATIENTS with Alzheimer's disease (AD) show loss of memory and cognitive deficits the molecular mechanisms of which are not completely known. We examined age-related changes in the expression levels of beta-amyloid precursor protein (beta APP) in the brain of the senescence accelerated mouse (SAM) P/10, which shows age-dependent brain atrophy and impairment in learning and memory, and in the senescence resistant mouse (SAM)-R/1 using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Levels of both beta APP mRNA and protein increased with age, reaching a peak at 8 months of age in the hippocampus of SAM-P/10. In contrast, beta APP protein level decreased with age in the hippocampus of SAM-R/1 while beta APP mRNA level did not change significantly. Levels of beta APP mRNA and protein showed no change with ageing in other brain regions, including cerebral cortex, thalamus/midbrain and cerebellum brain stem. These results suggest that the beta APP over-expression in the hippocampus might be related to the characteristic memory loss in SAM-P/10.