Simian foamy virus isolated from an accidentally infected human individual
Simian foamy virus isolated from an accidentally infected human individual
复制标题
DOI：
10.1128/jvi.71.6.4821-4824.1997
复制
发表时间：
1997-06-01
影响因子：
5.4
通讯作者：
NeumannHaefelin, D
中科院分区：
文献类型：
--
作者：
Schweizer, M;Falcone, V;NeumannHaefelin, D
人类天然泡沫病毒（FV）感染的证据仍然缺乏。然而，血清学和PCR已经证明人类意外感染猿猴FV，但之前在此类情况下回收传染性病毒的所有尝试都失败了。在这里，我们描述了从一个健康的动物看护人的外周血单核细胞（PBMC）中分离出一种猴FV，该动物看护人在20年前从非洲绿色猴（AGM）咬伤中获得了该病毒。人分离株的特性，如细胞培养物（包括人PBMC）中的宿主范围和在灵长类宿主中诱导中和抗体的能力，证明与从AGM获得的FV相似。发现分离株的基因组序列与宿主淋巴细胞中存在的前病毒序列几乎相同，与AGM分离株相关，但与实验室处理的所有FV分离株不同。为了成功地分离病毒，在与允许细胞共培养之前，用植物血凝素和白细胞介素-2刺激宿主淋巴细胞2周是必不可少的。与在FV感染的猴子中发现的情况相反，不可能从动物看护人的唾液中分离出病毒，也没有获得FV传播给家庭接触者的证据。我们的结论是，与猴子的主动感染相反，FV在人类意外感染后持续处于潜伏状态。
Evidence for natural foamy virus (FV) infections in humans is still lacking. However, accidental infections of humans with simian FV have been demonstrated by serology and PCR, but all previous attempts to recover infectious virus in such cases have failed. Here we describe the isolation of a simian FV from peripheral blood mononuclear cells (PBMC) of a healthy animal caretaker, who acquired the virus 20 gears ago from an African green monkey (AGM) bite. Properties of the human isolate such as host range in cell cultures including human PBMC and ability to induce neutralizing antibodies in the primate host proved to be similar to those of FV obtained from AGM. The genomic sequence of the isolate was found to be virtually identical to the proviral sequence present in the host lymphocytes and related to AGM isolates but distinct from those of all FV isolates handled in the laboratory. For successful virus isolation, it was essential to stimulate the host lymphocytes by phytohemagglutinin and interleukin-2 for 2 weeks prior to cocultivation with permissive cells. In contrast to the situation found in FV-infected monkeys, virus isolation from the saliva of the animal caretaker was not possible, and no evidence for FV transmission to family contacts was obtained. We conclude that, in contrast to active infection in monkeys, FV persists in a state of latency following accidental infection of humans.