Characterization of an AtCCX5 gene from Arabidopsis thaliana that involves in high-affinity K⁺ uptake and Na⁺ transport in yeast.
Characterization of an AtCCX5 gene from Arabidopsis thaliana that involves in high-affinity K⁺ uptake and Na⁺ transport in yeast.
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DOI：
10.1016/j.bbrc.2011.09.030
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发表时间：
2011-10
影响因子：
3.1
通讯作者：
Xinxin Zhang;M. Zhang;T. Takano;Shenkui Liu
中科院分区：
文献类型：
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作者：
Xinxin Zhang;M. Zhang;T. Takano;Shenkui Liu
从拟南芥中克隆了一个推定的阳离子钙交换剂（CCX）基因AtCCX5，并在酵母中分析了其功能。在酵母中表达的绿色荧光蛋白标记的AtCCX 5定位于质膜和核周围。制备表达AtCCX5的酵母转化体，并分析它们在各种阳离子（K+、Na+、Ca2+、Mg2+、Fe2+、Cu2+、Co2+、Cd2+、Mn2+、Ba2+、Ni2+、Zn2+和Li+）存在下的生长。AtCCX5的表达影响酵母对K+和Na+的反应。AtCCX5菌株对Zn2+的生长也稍好。表达AtCCX5的酵母突变体9.3在低K+（0.5mM）培养基上恢复了突变体的生长，并抑制了其Na+敏感性。离子吸收实验表明，AtCCX5介导相对高亲和力的K+吸收，也参与了Na+的运输酵母。综上所述，这些发现表明AtCCX5是一种新的转运蛋白，参与介导高亲和力的K+吸收和Na+的酵母运输。
The gene for a putative cation calcium exchanger (CCX) from Arabidopsis thaliana, AtCCX5, was cloned and its function was analyzed in yeast. Green fluorescent protein-tagged AtCCX5 expressed in yeast was localized in the plasma membrane and nuclear periphery. The yeast transformants expressing AtCCX5 were created and their growth in the presence of various cations (K+, Na+, Ca2+, Mg2+, Fe2+, Cu2+, Co2+, Cd2+, Mn2+, Ba2+, Ni2+, Zn2+, and Li+) were analyzed. AtCCX5 expression was found to affect the response to K+and Na+in yeast. The AtCCX5 transformant also showed a little better growth to Zn2+. The yeast mutant 9.3 expressing AtCCX5 restored growth of the mutant on medium with low K+(0.5mM), and also suppressed its Na+sensitivity. Ion uptake experiments showed that AtCCX5 mediated relatively high-affinity K+uptake and was also involved in Na+transport in yeast. Taken together, these findings suggest that the AtCCX5 is a novel transport protein involves in mediating high-affinity K+uptake and Na+transport in yeast.