Developmental and inducible accumulation of gene transcripts involved in alkaloid biosynthesis in opium poppy
Developmental and inducible accumulation of gene transcripts involved in alkaloid biosynthesis in opium poppy
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DOI：
10.1016/s0031-9422(03)00292-9
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发表时间：
2003-09-01
期刊：
影响因子：
3.8
通讯作者：
Park, SU
中科院分区：
文献类型：
--
作者：
Facchini, PJ;Park, SU
罂粟 (Papaver somniferum) 产生大量的苄基异喹啉生物碱，包括吗啡和血根碱，这些生物碱是通过分支点中间体 (S)-网状番荔枝碱从酪氨酸衍生而来。从罂粟中分离出参与 (S)-网状番荔枝碱生物合成的三种甲基转移酶：(S)-去甲乌贼碱-6-O-甲基转移酶 (6OMT)、(S)-3'-羟基-N-甲基乌贼碱-4'-O-甲基转移酶 (4'OMT) 和 (S)-乌贼碱 N-甲基转移酶 (CNMT)，并显示出具有广泛的同源性与来自日本黄连（Coptis japonica）的相应 cDNA。这些 cDNA 与先前分离的酪氨酸/多巴脱羧酶 (TYDC)、(S)-N-甲基椰壳碱-3'-羟化酶 (CYP80B1)、小檗碱桥酶 (BBE)、(7S)-salutaridinol 7-O-乙酰基转移酶 (SAT) 和可待因酮还原酶 (COR) 的克隆一起使用，以比较编码八种生物碱的基因转录本的积累罂粟中的生物合成酶。转录物水平通常在发育中的幼苗中增加，并且在茎和花蕾中始终较高，但在成熟植物的根和叶中变化更大。除 COR 外，每个转录本的积累都显示出对诱导剂处理或培养细胞损伤的显着诱导作用。特定基因转录水平通常与吗啡或血根碱的积累相关，但也有明显的例外。我们的数据表明罂粟生物碱生物合成基因的发育和诱导调节存在一定程度的协调。 (C) 2003 Elsevier Ltd. 保留所有权利。
Opium poppy (Papaver somniferum) produces a large number of benzylisoquinoline alkaloids, including morphine and sanguinarine, derived from tyrosine via the branch-point intermediate (S)-reticuline. Molecular clones for the three methlytransferases involved in (S)-reticuline biosynthesis, (S)-norcoclaurine-6-O-methyltransferase (6OMT), (S)-3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase (4'OMT), and (S)-coclaurine N-methyltransferase (CNMT), were isolated from opium poppy and shown to share extensive homology with the corresponding cDNAs from Japanese goldthread (Coptis japonica). These cDNAs were used together with previously isolated clones for tyrosine/dopa decarboxylase (TYDC), (S)-N-methylcoclaurine-3'-hydroxylase (CYP80B1), berberine bridge enzyme, (BBE), (7S)-salutaridinol 7-O-acetyltransferase (SAT), and codeinone reductase (COR), to compare the accumulation of gene transcripts encoding eight alkaloid biosynthetic enzymes in opium poppy. Transcript levels generally increased in developing seedlings and were consistently high in stems and flower buds, but were more variable in roots and leaves of mature plants. The accumulation of each transcript, with the exception of COR, showed a marked induction in response to elicitor treatment or wounding of cultured cells. Specific gene transcript levels often correlated with the accumulation of morphine or sanguinarine, with notable exceptions. Our data suggest some degree of coordination in the developmental and inducible regulation of alkaloid biosynthetic genes in opium poppy. (C) 2003 Elsevier Ltd. All rights reserved.