Identification of new netrin family members in zebrafish:: Developmental expression of netrin2 and netrin4
Identification of new netrin family members in zebrafish:: Developmental expression of netrin2 and netrin4
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DOI：
10.1002/dvdy.20474
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发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
Li, DY
中科院分区：
文献类型：
--
作者：
Park, KW;Urness, LD;Li, DY
Netrin 1是一种扩散因子，其将连合轴突吸引到脊髓的底板。最近的证据表明，Netrin 1广泛表达，并在多器官系统的发育中发挥作用。在哺乳动物中，有三个基因编码Netrins，而在斑马鱼中，只有Netrin 1的直系同源物netrin 1a和netrin 1b被确定。在这里，我们克隆了两个新的斑马鱼netrin，netrin 2和netrin 4，并提出了比较序列和表达分析。尽管与netrin 1a/netrin 1b具有显著的序列相似性，netrin 2显示出独特的表达模式。Netrin 2转录本首先在脊索和体节发生早期的体节中检测到。通过后期的体节发生，netrin 2在第四菱形节中表达，随后在后脑和耳泡中表达。相反，netrin 4在早期发育过程中仅以非常低的水平检测到。这四种Netrin的非重叠表达模式表明它们可能在斑马鱼发育中发挥独特的作用。
Netrin1 is a diffusible factor that attracts commissural axons to the floor plate of the spinal cord. Recent evidence indicates that Netrin1 is widely expressed and functions in the development of multiple organ systems. In mammals, there are three genes encoding Netrins, whereas in zebrafish, only the Netrin1 orthologs netrin1a and netrin1b have been identified. Here, we have cloned two new zebrafish Netrins, netrin2 and netrin4, and present a comparative sequence and expression analysis. Despite significant sequence similarity with netrin1a/netrin1b, netrin2 displays a unique expression pattern. Netrin2 transcript is first detected in the notochord and in developing somites at early somitogenesis. By late somitogenesis, netrin2 is expressed in the fourth rhombomere and is subsequently expressed in the hindbrain and otic vesicles. In contrast, netrin4 is detected only at very low levels during early development. The nonoverlapping expression patterns of these four Netrins suggest that they may play unique roles in zebrafish development.