Extraction optimization of polysaccharide from Zanthoxylum bungeanum using RSM and its antioxidant activity
Extraction optimization of polysaccharide from Zanthoxylum bungeanum using RSM and its antioxidant activity
复制标题
RSM法提取花椒多糖及其抗氧化活性
DOI：
10.1016/j.ijbiomac.2014.07.057
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发表时间：
2015-01-01
影响因子：
8.2
通讯作者：
Mao, Ziling
中科院分区：
文献类型：
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作者：
Li, Peiqin;Zhou, Ligang;Mao, Ziling
采用还原性三价铁、清除DPPH自由基、螯合亚铁和清除羟自由基等方法，对花椒果皮多糖进行体外抗氧化活性研究。在这四种抗氧化剂测定模型中，Z.花椒多糖（ZBP）具有较强的抗氧化活性，其EC 50值分别为0.011、0.021、0.056和0.008 mg/mL。并采用响应面法结合Box-Behnken试验设计进一步优化了ZBP的提取工艺。结果表明，在浸提温度89 ℃、浸提时间3 h、料液比29（v/w）、浸提次数2次的条件下，多糖得率最高，为13.96%，与预测的13.20%基本一致。本研究不仅为天然多糖抗氧化剂的开发提供了理论依据，而且为ZBP的规模化生产和进一步系统利用奠定了基础。(C)2014爱思唯尔有限公司版权所有。
Polysaccharide prepared from pericarp of Zanthoxylum bungeanum was proved to possess excellent antioxidant activities in vitro by using reducing ferric iron power, DPPH radical scavenging activity, chelating ferrous iron capacity, and hydroxyl radical scavenging activity assays in the present study. In those four antioxidant assay models, Z. bungeanum polysaccharide (ZBP) displayed prominent antioxidant activities with low EC50 values of 0.011, 0.021, 0.056 and 0.008 mg/mL, respectively. Moreover, the extraction process of ZBP was further optimized by response surface methodology combined with Box-Behnken design. The highest polysaccharide yield 13.96%, which agreed closely with the predicted yield 13.20%, was obtained under the optimal extraction conditions as follows: extraction temperature 89 degrees C, extraction time 3 h, ratio of water volume (mL) to raw material weight (g) 29 (v/w), and extraction number two times. The present research not only provide theoretical basis for exploitation of natural polysaccharide antioxidants, but also establish the foundation of large-scale production and further system utilization of ZBP. (C) 2014 Elsevier B.V. All rights reserved.