2 DISTINCT FACTORS ARE REQUIRED FOR INDUCTION OF T-CELL GROWTH
2 DISTINCT FACTORS ARE REQUIRED FOR INDUCTION OF T-CELL GROWTH
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DOI：
10.1038/283664a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
COUTINHO, A
中科院分区：
文献类型：
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作者：
LARSSON, EL;ISCOVE, NN;COUTINHO, A
T淋巴细胞活化的分子和细胞基础仍然是免疫学的中心问题。已知已经增殖的T细胞的生长依赖于T细胞生长因子（TCGF），一种生理性促分裂原1。然而，非循环T淋巴细胞对TCGF 2不敏感。它们需要与促有丝分裂凝集素（如伴刀豆球蛋白A（Con A）3或白细胞凝集素4）短暂接触，以结合并响应TCGF，然后只要TCGF不限制，它们将长期保持指数生长。虽然TCGF反应性的诱导是由于Con A与静息T细胞的直接接触3，但已知TCGF的凝集素依赖性产生涉及两种细胞类型，均存在于小鼠脾脏中1，3，6，7。一种由I-A阴性细胞组成，其中大多数是Thy-1阳性T淋巴细胞1，3，另一种由I-A阳性、免疫球蛋白阴性、Thy-1阴性细胞组成，其中大多数是巨噬细胞1，3。这两种细胞类型各自贡献的性质，特别是TCGF的细胞来源，尚未确定。我们现在已经确定了I-A阴性细胞群作为TCGF的来源，并在此表明巨噬细胞需要提供20，000分子量的因子，其在化学和功能上不同于TCGF，其支持I-A阴性细胞产生TCGF。
The molecular and cellular basis of T-lymphocyte activation remains a central question in immunology. The growth of already proliferating T cells is known to depend on T-cell growth factor (TCGF), a physiological mitogen1. Noncycling T lymphocytes, however, are not sensitive to TCGF2. They require a short contact with mitogenic lectins, such as concanavalin A (Con A)3or leukoagglutinin4to bind and respond to TCGF, and will thereafter maintain exponential growth for long periods provided that TCGF is not limiting5. While the induction of TCGF reactivity results from the direct contact of Con A with resting T cells3, the lectin-dependent production of TCGF is known to involve two cell types, both present in mouse spleen1,3,6,7. One consists of I- A-negative cells, most of which are Thy-1-positive T lymphocytes1,3, and the other consists of I-A-positive, immunoglobulin-negative, Thy-1-negative cells, most of which are macrophages1,3. The nature of the respective contributions of the two cell types, and in particular the cellular origin of TCGF, has not yet been established. We have now established the I-A-negative population as the source of TCGF and show here that macrophages are required to supply a 20,000-molecular weight factor, chemically and functionally distinct from TCGF, which supports the production of TCGF by the I-A-negative cells.