Antioxidant profiling of native Andean potato tubers (Solanum tuberosum L.) reveals cultivars with high levels of β-carotene, α-tocopherol, chlorogenic acid, and petanin
Antioxidant profiling of native Andean potato tubers (Solanum tuberosum L.) reveals cultivars with high levels of β-carotene, α-tocopherol, chlorogenic acid, and petanin
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DOI：
10.1021/jf0726583
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发表时间：
2007-12-26
影响因子：
6.1
通讯作者：
Larondelle, Yvan
中科院分区：
文献类型：
--
作者：
Andre, Christelle M.;Oufir, Mouhssin;Larondelle, Yvan
从人类营养的角度研究了23个安第斯马铃薯品种的抗氧化特性。主要的类胡萝卜素和生育酚化合物进行了研究，采用高效液相色谱法与二极管阵列检测器（HPLC-DAD）和荧光检测器，分别耦合，而多酚类物质（包括花色苷在有色块茎）的HPLC-MS和HPLC-DAD的装置进行了鉴定。抗氧化剂分析显示显着的基因型变异，以及从营养的角度来看，特别感兴趣的品种。促进健康的类胡萝卜素、叶黄素和玉米黄质的浓度范围为1.12至17.69 μ g g g（-1）干重（DW）和0至17.7 μ g g g（-1）干重，其中品种704353和702472分别显示出叶黄素和玉米黄质的最高水平。虽然β-胡萝卜素在马铃薯块茎中很少报道，但在16个本地品种中检测到这种膳食维生素原A类胡萝卜素的显着水平，范围为0.42至2.19 μ g g（-1）DW。在安第斯山脉马铃薯块茎中发现的α-生育酚含量从2.73到20.80微克/克干重不等，明显高于一般报道的商业品种的含量。绿原酸及其异构体占主导地位的多酚类化合物的每个品种。品种704429的深紫色肉质块茎含有极高水平的总花色苷（16.33 mg g（-1）DW），主要花色苷被鉴定为petanin（petunidin-3-p-coumaroyl-rutinoside-5-glucoside）。研究结果表明，安第斯马铃薯品种应利用筛选和育种计划的马铃薯品种的发展，增强健康和营养效益。
The antioxidant profile of 23 native Andean potato cultivars has been investigated from a human nutrition perspective. The main carotenoid and tocopherol compounds were studied using high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) and a fluorescence detector, respectively, whereas polyphenols (including anthocyanins in colored tubers) were identified by means of both HPLC-mass spectrometry and HPLC-DAD. Antioxidant profiling revealed significant genotypic variations as well as cultivars of particular interest from a nutritional point of view. Concentrations of the health-promoting carotenoids, lutein and zeaxanthin, ranged from 1.12 to 17.69 mu g g(-1) of dry weight (DW) and from 0 to 17.7 mu g g(-1) of DW, with cultivars 704353 and 702472 showing the highest levels in lutein and zeaxanthin, respectively. Whereas beta-carotene is rarely reported in potato tubers, remarkable levels of this dietary provitamin A carotenoid were detected in 16 native varieties, ranging from 0.42 to 2.19 mu g g(-1) of DW. The amounts of alpha-tocopherol found in Andean potato tubers, extending from 2.73 to 20.80 mu g g(-1) of DW, were clearly above the quantities generally reported for commercial varieties. Chlorogenic acid and its isomers dominated the polyphenolic profile of each cultivar. Dark purple-fleshed tubers from the cultivar 704429 contained exceptionally high levels of total anthocyanins (16.33 mg g(-1) of DW), The main anthocyanin was identified as petanin (petunidin-3-p-coumaroyl-rutinoside-5-glucoside). The results suggest that Andean potato cultivars should be exploited in screening and breeding programs for the development of potato varieties with enhanced health and nutritional benefits.