Biotic Elicitors Effectively Increase the Glucosinolates Content in Brassicaceae Sprouts
Biotic Elicitors Effectively Increase the Glucosinolates Content in Brassicaceae Sprouts
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DOI：
10.1021/jf404876z
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发表时间：
2014-02-26
影响因子：
6.1
通讯作者：
Moreno, Diego A.
中科院分区：
文献类型：
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作者：
Baenas, Nieves;Garcia-Viguera, Cristina;Moreno, Diego A.
几种生物诱导子已被用于菊科植物中以提高其植物化学品质。然而，在受控生长条件下，激发子之间没有比较。为了得出关于使用激发子来富集即食豆芽中促进健康的硫代葡萄糖苷的一般结论，本研究的目的是揭示植物激素茉莉酸甲酯（25 μ M）、茉莉酸（150 μ M）和水杨酸（100 μ M）、低聚糖葡萄糖（277 mM）和蔗糖（146 mM）以及氨基酸DL-蛋氨酸（5 mM）作为诱导子在8天发芽甘蓝中的作用（花椰菜）、甘蓝型油菜（芜菁甘蓝）、芜菁（芜菁）和萝卜（中国玫瑰萝卜和红萝卜），它们是先前研究的具有高硫代葡萄糖苷的代表性物种。结果表明，植物激素茉莉酸甲酯、茉莉酸和糖类是有效的诱导剂，能提高豆芽中硫代葡萄糖苷的含量，尤其是萝卜硫苷的含量（在MeJA处理的西兰花芽中为183至294 mg. 100 g（-1）），萝卜甙（MeJA处理的月季萝卜和红萝卜分别为33 - 124 mg·100 g（-1）和167 - 227 mg·100 g（-1）），和葡萄糖巴西菌素（在MeJA处理的芜菁和芜菁甘蓝芽中分别为23.4 - 91.0 mg·100 g（-1）和29.6 - 186 mg·100 g（-1））。
Several biotic elicitors have been used in Brassicaceae species to enhance their phytochemical quality. However, there is no comparison between elicitors under controlled growth conditions. In order to draw general conclusions about the use of elicitors to enrich ready-to-eat sprouts in health-promoting glucosinolates, the aim of this study was to unveil the effect of the phytohormones methyl jasmonate (25 mu M), jasmonic acid (150 mu M), and salicylic acid (100 mu M), the oligosaccharides glucose (277 mM) and sucrose (146 mM), and the amino acid DL-methionine (5 mM) as elicitors over 8-day sprouting Brassica oleraceae (broccoli), Brassica napus (rutabaga cabbage), Brassica rapa (turnip), and Raphanus sativus (China rose radish and red radish), representative species high in glucosinolates previously studied. Results indicated that the phytohormones methyl jasmonate and jasmonic acid and the sugars acted as effective elicitors, increasing the total glucosinolate contents of the sprouts, particularly, glucoraphanin (from 183 to 294 mg.100 g(-1) in MeJA-treated broccoli sprouts), glucoraphenin (from 33 to 124 mg.100 g(-1) and from 167 to 227 mg.100 g(-1) in MeJA-treated China rose radish and red radish, respectively), and glucobrassicin (from 23.4 to 91.0 mg.100 g(-1) and from 29.6 to 186 mg.100 g(-1) in MeJA-treated turnip and rutabaga sprouts, respectively).