Molecular assessment of bacterial vaginosis by Lactobacillus abundance and species diversity.
Molecular assessment of bacterial vaginosis by Lactobacillus abundance and species diversity.
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DOI：
10.1186/s12879-016-1513-3
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发表时间：
2016-04-23
影响因子：
3.7
通讯作者：
Kort R
中科院分区：
文献类型：
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作者：
Dols JA;Molenaar D;van der Helm JJ;Caspers MP;de Kat Angelino-Bart A;Schuren FH;Speksnijder AG;Westerhoff HV;Richardus JH;Boon ME;Reid G;de Vries HJ;Kort R
到目前为止，妇女最常被诊断为细菌性阴道病(BV)，使用基于显微镜的Nugent评分或Amsel标准。然而，精度并不是最优的。本研究的目的是确认已知的BV相关成分图谱的一致性，并使用三种分子方法评估BV的指标。对BV指标的评估是通过对V5-V7区的16S rRNA扩增序列、定制的基于16S rRNA寡核苷酸的微阵列和基于聚合酶链式反应的分析技术进行的，该技术基于16S-23S rRNA基因间隔区的片段可变性。对40名在荷兰性传播感染门诊就诊的女性进行了阴道细菌种类的调查，其中20人被诊断为BV(Nugent评分7-10)，20名BV阴性(Nugent评分0-3)。16S rRNA扩增序列分析显示，在BV阴性的妇女中有两个菌群，其中以乳杆菌或皱纹乳杆菌为主，在BV阳性的妇女中有三个不同的菌群。在前者中，皱纹夜蛾和细纹夜蛾之间存在几乎完全的负相关。BV阳性患者的菌种多样性较高，且以阴道加德纳氏菌、细毛菌科和阴道加德纳氏菌为主。因此，物种多样性的Gini-Simpson指数和乳杆菌的相对丰度对BV的影响是一致的。在所使用的条件下，只有16S rRNA扩增片段测序法适合于物种多样性的评估，而三种分子组成谱方法都能够指示乳杆菌在阴道微生物区系中的丰度。一种负担得起的简单的分子检测表明乳杆菌属的枯竭，结合阴道微生物区系物种多样性的增加，可以作为评估BV的一种替代和实用的诊断方法。本文的在线版本(doi：10.1186/s12879-0161513-3)包含补充材料，授权用户可以使用。
To date, women are most often diagnosed with bacterial vaginosis (BV) using microscopy based Nugent scoring or Amsel criteria. However, the accuracy is less than optimal. The aim of the present study was to confirm the identity of known BV-associated composition profiles and evaluate indicators for BV using three molecular methods. Evaluation of indicators for BV was carried out by 16S rRNA amplicon sequencing of the V5-V7 region, a tailor-made 16S rRNA oligonucleotide-based microarray, and a PCR-based profiling technique termed IS-profiling, which is based on fragment variability of the 16S-23S rRNA intergenic spacer region. An inventory of vaginal bacterial species was obtained from 40 females attending a Dutch sexually transmitted infection outpatient clinic, of which 20 diagnosed with BV (Nugent score 7–10), and 20 BV negative (Nugent score 0–3). Analysis of the bacterial communities by 16S rRNA amplicon sequencing revealed two clusters in the BV negative women, dominated by either Lactobacillus iners or Lactobacillus crispatus and three distinct clusters in the BV positive women. In the former, there was a virtually complete, negative correlation between L. crispatus and L. iners. BV positive subjects showed cluster profiles that were relatively high in bacterial species diversity and dominated by anaerobic species, including Gardnerella vaginalis, and those belonging to the Families of Lachnospiraceae and Leptotrichiaceae. Accordingly, the Gini-Simpson index of species diversity, and the relative abundance Lactobacillus species appeared consistent indicators for BV. Under the conditions used, only the 16S rRNA amplicon sequencing method was suitable to assess species diversity, while all three molecular composition profiling methods were able to indicate Lactobacillus abundance in the vaginal microbiota. An affordable and simple molecular test showing a depletion of the genus Lactobacillus in combination with an increased species diversity of vaginal microbiota could serve as an alternative and practical diagnostic method for the assessment of BV. The online version of this article (doi:10.1186/s12879-016-1513-3) contains supplementary material, which is available to authorized users.