Glucose enzyme electrode using cytochrome b562 as an electron mediator
Glucose enzyme electrode using cytochrome b562 as an electron mediator
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DOI：
10.1016/s0956-5663(03)00037-x
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发表时间：
2003-05-01
影响因子：
12.6
通讯作者：
Sode, K
中科院分区：
文献类型：
--
作者：
Okuda, J;Wakai, J;Sode, K
我们展示了使用来自醋酸钙不动杆菌的吡咯喹啉醌 (PQQ) 葡萄糖脱氢酶 (PQQGDH) 和来自黑曲霉的葡萄糖氧化酶 (GOD) 以及大肠杆菌可溶性细胞色素 b(562) (cyt b(562)) 作为电子受体的葡萄糖传感器的构建。 PQQGDH 和 GOD 没有表现出电极表面辅基的直接电化学循环，从而产生相应的电流信号。我们构建了与 100 倍摩尔过量的 cyt b(562) 共固定的 PQQGDH 和 GOD 电极，并在没有合成电子介体的情况下研究了电化学性能。 PQQGDH/cyt b(562) 和 GOD/cyt b(562) 电极均对葡萄糖响应良好，而单独固定酶的电极未观察到电流增加。 PQQGDH/cyt b(562) 和 GOD/cyt b(562) 电极的检测限分别为 0.1 和 0.8 mM，并且它们的线性分别延伸至超过 2 和 9 mM。这些结果表明，可以通过使用天然电子受体在没有合成电子介体的情况下构建传感器系统。此外，我们还证明了 cyt b(562) 在具有四级结构不包含任何电子转移亚基的氧化还原酶的直接电子转移型传感器系统中的潜在应用。 (C) 2003 Elsevier Science B.V. 保留所有权利。
We demonstrate the construction of glucose sensors employing pyrroloquinoline quinone (PQQ) glucose dehydrogenase (PQQGDH) from Acinetobacter calcoaceticus and glucose oxidase (GOD) from Aspergillus nigar coupled with Escherichia coli soluble cytochrome b(562) (cyt b(562)) as electron acceptor. PQQGDH and GOD do not show direct electrochemical recycling of the prosthetic group at the electrode surface leading to a corresponding current signal. We constructed PQQGDH and GOD electrodes co-immobilized with 100-fold molar excess of cyt b(562) and investigated the electrochemical properties without synthetic electron mediators. PQQGDH/cyt b(562) and GOD/cyt b(562) electrodes both responded well to glucose whereas no current increase was observed from the electrode immobilizing enzyme alone. The detection limits for the PQQGDH/cyt b(562) and GOD/cyt b(562) electrodes were 0.1 and 0.8 mM, respectively, and their linearity extended to over 2 and 9 mM, respectively. These results demonstrate that a sensor system can be constructed without a synthetic electron mediator by using a natural electron acceptor. Furthermore, we have demonstrated the potential application of cyt b(562) in direct electron transfer type sensor systems with oxidoreductases whose quaternary structure do not contain any electron transfer subunit. (C) 2003 Elsevier Science B.V. All rights reserved.