Structural characterization and in vitro inhibitory activities in P-selectin-mediated leukocyte adhesion of polysaccharide fractions isolated from the roots of Physalis alkekengi
Structural characterization and in vitro inhibitory activities in P-selectin-mediated leukocyte adhesion of polysaccharide fractions isolated from the roots of Physalis alkekengi
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从酸浆根中分离的多糖组分的结构表征和 P-选择素介导的白细胞粘附的体外抑制活性
DOI：
10.1016/j.ijbiomac.2011.04.015
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发表时间：
2011-08-01
影响因子：
8.2
通讯作者：
Zeng, Xianlu
中科院分区：
文献类型：
--
作者：
Tong, Haibin;Wang, Ruifei;Zeng, Xianlu
选择素介导的白细胞初始附着和在血管内皮表面的滚动是炎症反应的关键步骤。由于p -选择素是抗炎症治疗策略的一个有前景的靶点，近年来的研究工作主要集中在各种天然碳水化合物中寻找更有效和无毒的p -选择素拮抗剂。本研究采用deae -纤维素和sepphacryl S-200层析法从酸浆根中分离得到3个水溶性多糖（PPS-1、PPS-2和PPS-3）。采用化学方法、气相色谱法、高效液相色谱法、傅里叶变换红外光谱法、部分酸水解法、甲基化法和气相色谱-质谱法对其进行了理化和结构表征。通过流式细胞术、静态粘附和层流实验评价多糖组分对p选择素介导的白细胞粘附的抑制能力。结果表明，不同的多糖组分在碳水化合物、蛋白质和醛酸含量、分子量、单糖组成和糖苷连锁类型等方面具有不同的理化和结构性质。多糖组分中，PPS-2能有效阻断p -选择素与其天然配体的相互作用。(C) 2011 Elsevier B.V.版权所有
Selectin-mediated leukocyte initial attachment and rolling over vessel endothelial surface are crucial steps for inflammatory responses. As P-selectin is a promising target for anti-inflammation therapeutic strategy, recent works have focused on searching for more potent and non-toxic P-selectin antagonists among various natural carbohydrate products. Here, we isolated three water-soluble polysaccharide fractions (PPS-1, PPS-2 and PPS-3) from the roots of Physalis alkekengi by DEAE-cellulose and Sephacryl S-200 chromatography. Their physicochemical and structural characterizations were determined by chemical methods, GC (gas chromatography), HPLC (high performance liquid chromatography), FTIR (Fourier transform infrared spectrometry), partial acid hydrolysis, methylation and GC-MS (gas chromatography-mass spectrometry) analyses. The inhibitory capacity of the polysaccharide fractions in P-selectin-mediated leukocyte adhesion was evaluated by flow cytometric, static adhesion and laminar flow assays. Results showed that different polysaccharide fractions possess distinct physicochemical and structural properties, including carbohydrate, protein and uronic acid contents, molecular weight, monosaccharide composition and glycosidic linkage type. Among the polysaccharide fractions, PPS-2 could effectively block the interaction between P-selectin and its native ligand. (C) 2011 Elsevier B.V. All rights reserved.