LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1
LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1
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DOI：
10.2147/cmar.s234891
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Wu, Dabao
中科院分区：
文献类型：
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作者：
Ke, Jieqi;Shen, Zheng;Wu, Dabao
简介：DCST1-AS1 的功能已在肝癌中得到研究，但其在子宫内膜癌 (EC) 中的作用仍知之甚少。本研究旨在分析DCST1-AS1在EC中的作用。方法：从62例EC患者中获得配对的EC和非肿瘤组织样本。这些患者自入院以来进行了5年的随访，记录他们的生存状况。 HEC-1 细胞用 DCST1-AS1、Notch1 载体、miRNA 阴性对照或 miR-92a-3p 模拟物转染。测量荧光素酶活性。应用QPCR和Western blot分别测定RNA水平和蛋白表达。采用Transwell实验分析HEC-1细胞的侵袭和迁移能力。结果：本研究发现DCST1-AS1在EC中表达上调。生存分析显示，高水平的 DCST1-AS1 表达预示 EC 患者的生存率较差。生物信息学分析显示miR-92a-3p可能结合DCST1-AS1，并且双荧光素酶活性测定进一步证实了两者之间的相互作用。然而，miR-92a-3p和DCST1-AS1的过表达未能影响彼此的表达。此外，DCST1-AS1过度表达导致Notch1上调并增加癌细胞侵袭和迁移率。 miR-92a-3p的过表达起到相反的作用，减弱了DCST1-AS1过表达的影响。讨论：DCST1-AS1在EC中下调，可能海绵miR-92a-3p，从而促进癌细胞侵袭和迁移。
Introduction: The functions of DCST1-AS1 have been investigated in liver cancer, while its role in endometrial carcinoma (EC) remains hardly known. This study aimed to analyze the role of DCST1-AS1 in EC.Methods: Paired EC and non-tumor tissue samples were obtained from 62 EC patients. These patients were followed up for 5 years since their admission to record their survival conditions. HEC-1 cells were transfected with DCST1-AS1, Notch1 vectors, miRNA negative control or miR-92a-3p mimic. Luciferase activity was measured. QPCR and Western blot were applied to determine the RNA level and protein expression, respectively. The invasion and migration of HEC-1 cells were analyzed by Transwell assay.Results: We in this study found that DCST1-AS1 was upregulated in EC. Survival analysis revealed that high levels of DCST1-AS1 expression predicted poor survival of EC patients. Bioinformatics analysis revealed that miR-92a-3p may bind DCST1-AS1 and the interaction between them was further confirmed by dual-luciferase activity assay. However, overexpression of miR-92a-3p and DCST1-AS1 failed to affect the expression of each other. Moreover, DCST1-AS1 overexpression led to upregulated Notch1 and increased cancer cell invasion and migration rates. Overexpression of miR-92a-3p played an opposite role and attenuated the effects of DCST1-AS1 overexpression.Discussion: DCST1-AS1 is downregulated in EC and may sponge miR-92a-3p, thereby promoting cancer cell invasion and migration.