Global analysis of the meiotic crossover landscape
Global analysis of the meiotic crossover landscape
复制标题
DOI：
10.1016/j.devcel.2008.07.006
复制
发表时间：
2008-09-16
影响因子：
11.8
通讯作者：
Fung, Jennifer C.
中科院分区：
文献类型：
--
作者：
Chen, Stacy Y.;Tsubouchi, Tomomi;Fung, Jennifer C.
严格控制交换的数量和分布对减数分裂是非常重要的。交叉建立交叉，交叉是同源染色体之间的物理连接，提供了在减数分裂纺锤体上排列染色体所必需的张力。理解交叉控制的机制一直受到交叉分布难以确定的阻碍。在这里，我们提出了一种基于微阵列的方法来分析交叉控制的多个方面，同时快速，高分辨率，全基因组，并在细胞的基础上。使用这种方法，我们表明，在zip 2和zip 4/spo 22突变体的干扰损失是伴随着减少交叉稳态，从而连接这两个水平的交叉控制。我们还提供了证据表明，抑制在端粒和着丝粒的交叉产生不同的机制。最后，我们发现了一个令人惊讶的作用，突触复合体组件Zip 1在抑制交叉在着丝粒。
Tight control of the number and distribution of crossovers is of great importance for meiosis. Crossovers establish chiasmata, which are physical connections between homologous chromosomes that provide the tension necessary to align chromosomes on the meiotic spindle. Understanding the mechanisms underlying crossover control has been hampered by the difficulty in determining crossover distributions. Here, we present a microarray-based method to analyze multiple aspects of crossover control simultaneously and rapidly, at high resolution, genome-wide, and on a cell-by-cell basis. Using this approach, we show that loss of interference in zip2 and zip4/spo22 mutants is accompanied by a reduction in crossover homeostasis, thus connecting these two levels of crossover control. We also provide evidence to suggest that repression of crossing over at telomeres and centromeres arises from different mechanisms. Lastly, we uncover a surprising role for the synaptonemal complex component Zip1 in repressing crossing over at the centromere.