Biopotency of α-Tocopherols as Determined by Curative Myopathy Bioassay in the Rat
Biopotency of α-Tocopherols as Determined by Curative Myopathy Bioassay in the Rat
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通过大鼠治疗性肌病生物测定法测定 α-生育酚的生物效力
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
M. Brin
中科院分区：
文献类型：
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作者：
L. Machlin;E. Gabriel;M. Brin
维生素E缺乏大鼠给予各种生育酚后血浆丙酮酸激酶活性的降低为维生素E活性的三点平行斜率测定提供了基础。6次重复试验表明，rrr - α -生育酚乙酸酯（rrr - α - ta）的活性是all-rac- α - ta的141%。这证实了许多其他生物测定的先前报告，并且与国家处方集指定的单位重量关系一致。2-氨基- α -生育酚乙酸酯的活性是all-rac- α - ta的97%，这表明仅在铬酚环上2位的构型对确定α -生育酚的生物活性是重要的。
: The decrease in plasma pyruvate kinase activity after administration of various tocopherols to vitamin E-deficient rats provided the basis for a 3-point parallel-slope assay for vitamin E activity. Based on 6 replicate assays, RRR-alpha-tocopheryl acetate (RRR-alpha-TA) was 141% as active as all-rac-alpha-tocopheryl acetate (all-rac-alpha-TA). This confirms many previous reports with other bioassays and is in agreement with the unit-weight relationships assigned by The National Formulary. 2-ambo-alpha-Tocopheryl acetate was 97% as active as all-rac-alpha-TA indicating that only the configuration of the 2-position on the chromanol ring is important in determining the biological activity of alpha-tocopherol.