Morphologic characterization of osteosarcoma growth on the chick chorioallantoic membrane.
Morphologic characterization of osteosarcoma growth on the chick chorioallantoic membrane.
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DOI：
10.1186/1756-0500-3-58
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发表时间：
2010-03-04
影响因子：
1.8
通讯作者：
Hagedorn M
中科院分区：
文献类型：
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作者：
Balke M;Neumann A;Kersting C;Agelopoulos K;Gebert C;Gosheger G;Buerger H;Hagedorn M
鸡绒毛膜-尿囊膜（CAM）试验是研究体内血管生成或抗血管生成活性的常用方法。通过生物显微镜直接监测肿瘤生长的方便性以及以一种廉价的方式筛选许多样本的可能性。CAM模型为研究干扰生理性血管生成或源自癌细胞系的实验性肿瘤的分子效应提供了一个强有力的工具。因此，我们筛选了8个骨肉瘤细胞系，看它们在CAM上形成血管化肿瘤的能力。我们在胚胎发育第10天将300万~ 500万个人骨肉瘤细胞系（HOS、MG63、MNNG-HOS、OST、SAOS、SJSA1、U2OS、ZK58）植入CAM。在不同时间点用体内生物显微镜观察肿瘤的生长情况，并在细胞移植后7天将肿瘤固定在多聚甲醛中。组织观察，拍照和选择的病例进一步分析使用标准组织学。从8个细胞系中，MNNG-HOS、U2OS和SAOS移植到CAM上后能够形成实体瘤。MNNG-HOS肿瘤表现出最可靠和一致的生长，能够穿透绒毛膜上皮，在CAM基质中生长，并诱导强烈的血管生成反应。我们的研究结果表明，CAM实验是研究骨肉瘤生长的一个有用的工具。该模型为目前的啮齿动物模型提供了一个很好的替代方案，可以作为抗癌分子的临床前筛选试验。这可能会加快治疗骨肉瘤新药的开发速度和疗效。
The chick chorio-allantoic membrane (CAM) assay is a commonly used method for studying angiogenic or anti-angiogenic activities in vivo. The ease of access allows direct monitoring of tumour growth by biomicroscopy and the possibility to screen many samples in an inexpensive way. The CAM model provides a powerful tool to study effects of molecules, which interfere with physiological angiogenesis, or experimental tumours derived from cancer cell lines. We therefore screened eight osteosarcoma cell lines for their ability to form vascularized tumours on the CAM. We implanted 3-5 million cells of human osteosarcoma lines (HOS, MG63, MNNG-HOS, OST, SAOS, SJSA1, U2OS, ZK58) on the CAM at day 10 of embryonic development. Tumour growth was monitored by in vivo biomicroscopy at different time points and tumours were fixed in paraformaldehyde seven days after cell grafting. The tissue was observed, photographed and selected cases were further analyzed using standard histology. From the eight cell lines the MNNG-HOS, U2OS and SAOS were able to form solid tumours when grafted on the CAM. The MNNG-HOS tumours showed the most reliable and consistent growth and were able to penetrate the chorionic epithelium, grow in the CAM stroma and induce a strong angiogenic response. Our results show that the CAM assay is a useful tool for studying osteosarcoma growth. The model provides an excellent alternative to current rodent models and could serve as a preclinical screening assay for anticancer molecules. It might increase the speed and efficacy of the development of new drugs for the treatment of osteosarcoma.