Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
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DOI：
10.1117/1.1586704
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发表时间：
2003-07-01
影响因子：
3.5
通讯作者：
Ruan, QQ
中科院分区：
文献类型：
--
作者：
Gratton, E;Breusegem, S;Ruan, QQ
在激光扫描显微镜上用时间相关单光子计数法和频域法获得了荧光寿命图像。在同一个显微镜系统中，我们实现了这两种方法。我们执行的两种方法的性能进行比较的信噪比（SNR）和数据采集的速度。虽然在我们的实际实现中，时间相关单光子计数技术为低强度图像提供了更好的SNR，但频域方法更快，并且为明亮样本提供了更少的失真。(C)2003年，任光化学学会理事。
Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples. (C) 2003 Society of Photo-Optical.