Heparin alters the expression of different forms of immunoglobulin mu heavy chains and their associated proteins by pre-B cell lines and normal Ly-1 (CD5+) B cells.
Heparin alters the expression of different forms of immunoglobulin mu heavy chains and their associated proteins by pre-B cell lines and normal Ly-1 (CD5+) B cells.
复制标题
肝素改变前 B 细胞系和正常 Ly-1 (CD5) B 细胞不同形式的免疫球蛋白 mu 重链及其相关蛋白的表达。
DOI：
10.1093/intimm/3.11.1117
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发表时间：
1991
影响因子：
4.4
通讯作者：
Herzenberg,LA
中科院分区：
文献类型：
--
作者：
Chen,J;Herzenberg,LA;Herzenberg,LA
本文介绍的研究表明，肝素可改变鼠前B细胞系和正常Ly-1（CD 5+）B细胞的免疫球蛋白表达。先前的研究表明，前B细胞系70 Z/3和NFS-5.3.express μ重链存在于细胞质中并有少量存在于细胞表面。这些细胞质和表面μ均与ω（λ5）替代轻链二硫键连接，并与1（Vpre-B）可变区样蛋白非共价结合。我们表明，用肝素培养70 Z/3减少了细胞表面膜形式μ（μm）的量。与肝素一起培养NFS-5.3同样降低了膜形式μ;然而，它增加了含有分泌形式μ（μS）重链、二硫键连接的ω（λ5）链和非共价结合蛋白质的五元μi分子的表面水平。用肝素培养腹膜B细胞也增加了分泌型μS的产生，在这种情况下，通过分泌经典的五倍IgM可以检测到μS。类似地，腹膜内注射肝素增加腹膜Ly-1 B细胞的IgM分泌。肝素可影响前B细胞和B细胞的分化和功能。
Studies presented here show that heparin alters immunoglobulin expression by murine pre-B cell lines and normal Ly-1 (CD5+) B cells. Previous studies have shown that pre-B cell lines 70Z/3 and NFS-5.3.express μ heavy chains in the cytoplasm and a small amount on the cell surface. Both these cytoplasmic and surface μ are disulfide-linked to ω(λ5) surrogate light chains and are noncovalently associated with1(Vpre-B) variable region-like proteins. We show that culturing 70Z/3 with heparin reduces the amount of the membrane-form μ (μm) on the cell surface. Culturing NFS-5.3 with heparin similarly decreases the membrane-form μ; however, it increases the surface level of a pentametric μi molecule containing secreted-form μ(μS) heavy chains, disulfide-linked ω (λ5) chains, and noncovalently associated proteins. Culturing peritoneal B cells with heparin also increases the production of the secreted-form μS, detectable in this case by the secretion of classical pentametric IgM. Similarly, injecting heparin intraperitoneally increases IgM secretion by peritoneal Ly-1 B cells. Thus heparin could influence pre-B cell and B cell differentiation and function.