A NEW ANIMAL-MODEL OF ATRIAL-FLUTTER
A NEW ANIMAL-MODEL OF ATRIAL-FLUTTER
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DOI：
10.1016/s0735-1097(86)80429-6
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发表时间：
1986-10-01
影响因子：
24
通讯作者：
WALDO, AL
中科院分区：
文献类型：
--
作者：
PAGE, PL;PLUMB, VJ;WALDO, AL
本文建立了一种新的、简单可靠的心包炎心包切开术后心房扑动模型。采用无菌技术，通过右开胸打开心包，将涂有聚四氟乙烯的不锈钢丝电极固定在心房选定的三个位置并将其外化，在心房表面大量涂抹滑石粉，并在游离的左右心房壁上放置单层纱布。这些狗被允许康复。随后，在有意识的非镇静状态下，通过每日程序性心房刺激研究确定心房扑动的诱导性和心房的选定电生理特性。最初研究的25只狗中有23只可诱发心房扑动。它是持续的（即持久的）。23次中有17次（5分钟）。心房兴奋性、房内传导时间和心房难治性均不能通过起搏和三个固定位置记录来预测心房扑动的诱发性。分析了23只狗诱发的139次心房扑动。平均持续心房扑动周期长度为131±。20毫秒(平均+-。SD)（范围100至170）；心房扑动周期长度≥150ms的有23次，≥120 ~ 150ms的有64次，≤20ms的有52次。在5只狗中，研究了持续心房扑动期间心房扑动周期长度的稳定性，结果表明，在诱导后35至95分钟被快速心房起搏中断之前，所有5只狗的心房扑动周期长度都非常稳定。17只犬再次手术进行心外膜定位，其中12只犬在开胸状态下可诱发心房扑动。总之，该无菌心包炎犬心脏心房扑动模型可靠性高，可重复性好，易于创建。
A new, simple and reliable model of atrial flutter utilizing postpericardiotomy pericarditis was developed in the dog. Using a sterile technique, the pericardium was opened by way of a right thoracotomy, Teflon-coated, stainless steel wire electrodes were fixed to three selected sites on the atria and exteriorized, the atrial surfaces were generously dusted with talcum powder and a single layer of gauze was placed on the free left and right atrial walls. The dogs were allowed to recover. Subsequently, the inducibility of atrial flutter and selected electrophysiologic properties of the atria were determined by daily programmed atrial stimulation studies with the dogs in the conscious, nonsedated state. Atrial flutter could be induced in 23 of 25 dogs initially studied. It was sustained (that is, lasting .gtoreq.5 min) in 17 of the 23. Neither atrial excitability, intraatrial conduction time nor atrial refractoriness determined by the pacing and recording from the three fixed sites predicted the inducibility of atrial flutter. One hundred thirty-nine episodes of atrial flutter induced in these 23 dogs were analyzed. The mean sustained atrial flutter cycle length was 131 .+-. 20 ms (mean .+-. SD) (range 100 to 170); the atrial flutter cycle length was 150 ms or more in 23 episodes, between 120 and 150 ms in 64 episodes and 20 ms or less in 52 episodes. In five dogs, the stability of the atrial flutter cycle length during sustained atrial flutter was studied and shown to be remarkably stable in all five until interrupted by rapid atrial pacing 35 to 95 minutes after its induction. Seventeen dogs were submitted to reoperation for epicardial mapping purposes and atrial flutter could be induced in the open chest state in 12. In conclusion, this sterile pericarditis model of atrial flutter in the canine heart proved to be highly reliable, reproducible and easy to create.