Sustained inhibition of corneal neovascularization by genetic ablation of CCR5
Sustained inhibition of corneal neovascularization by genetic ablation of CCR5
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DOI：
10.1167/iovs.02-0685
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发表时间：
2003-02-01
影响因子：
4.4
通讯作者：
Ambati, J
中科院分区：
文献类型：
--
作者：
Ambati, BK;Anand, A;Ambati, J
PURPOSE To determine whether genetic ablation of the CC chemokine receptor CCR5 (involved in leukocyte and endothelial chemotaxis) inhibits the development of corneal neo-vascularization.METHODS. Wild-type C57BL/6J mice and species-specific counterparts with targeted homozygous disruption of the CCR5 gene underwent chemical and mechanical denudation of corneal and limbal epithelium. Corneas were harvested 2 and 4 weeks after injury. Neovascularization was quantified by CD31 immunostaining. Expression of VEGF protein was quantified by ELSIA.RESULTS. The mean percentages of neovascularized corneal area in control mice and CCR5-deficient truce, 2 weeks after denudation were 58.3%. and 38.5% (P = 0.05), respectively, At 4 weeks after denudation, the corresponding percentages were 67.6% and 44.0% (P = 0.028). In CCR5-deficient mice, VEGF protein levels were reduced 51.1% at 2 weeks (P = 0.05) after injury and 37.3% at 4 weeks (P = 0.03)CONCLUSIONS. CCR5-deficient mice showed a persistent 34% to 35% inhibition of corneal neovascularization for tip to 4 weeks. This inhibition correlates with reduced expression of VEGF. These data implicate CCR5 as one essential component in the development of corneal neovascularization.
