Direct magnetic resonance imaging of histological tissue samples at 3.0T
Direct magnetic resonance imaging of histological tissue samples at 3.0T
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DOI：
10.1002/mrm.21213
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Yang, Qing X.
中科院分区：
文献类型：
--
作者：
Meadowcroft, Mark D.;Zhang, Shutong;Yang, Qing X.
组织切片的直接成像具有挑战性。组织切片的平面尺寸和厚度之间的尺寸的巨大差异将需要RF线圈在具有最小厚度的2D平面中产生均匀的RF磁场（EII）。在这项工作中，开发和测试了一种专门用于组织切片成像的新型RF线圈。实验数据表明，线圈是高度敏感的，并能够产生一个均匀的B，场分布在一个平面区域的组织学载玻片，允许高分辨率的T-2图像和T-2地图从60 μ m厚的组织学样本的采集。将图像强度和T2分布与同一切片的相对铁浓度的组织学染色直接进行比较。这项工作证明了使用显微成像组织学线圈对病理病变组织的薄片进行成像以获得染色组织和MR图像之间精确的一对一比较的可行性。
Direct imaging of a histological slice is challenging. The vast difference in dimension between planar size and the thickness of histology slices would require an RF coil to produce a uniform RF magnetic (Ell) field in a 2D plane with minimal thickness. In this work a novel RF coil designed specifically for imaging a histology slice was developed and tested. The experimental data demonstrate that the coil is highly sensitive and capable of producing a uniform B, field distribution in a planar region of histological slides, allowing for the acquisition of high-resolution T-2 images and T-2 maps from a 60-mu m-thick histological sample. The image intensity and T2 distributions were directly compared with histological staining of the relative iron concentration of the same slice. This work demonstrates the feasibility of using a microimaging histological coil to image thin slices of pathologically diseased tissue to obtain a precise one-to-one comparison between stained tissue and MR images.