Ensheathing cells: Large scale purification from adult olfactory bulb, freeze-preservation and migration of transplanted cells in adult brain
Ensheathing cells: Large scale purification from adult olfactory bulb, freeze-preservation and migration of transplanted cells in adult brain
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DOI：
10.3233/rnn-1996-10104
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发表时间：
1996-07-01
影响因子：
2.8
通讯作者：
NietoSampedro, M
中科院分区：
文献类型：
--
作者：
GudinoCabrera, G;NietoSampedro, M
再生的神经纤维芽包裹嗅球（OB）成鞘细胞（ECs）似乎逃脱了胶质组织的抑制影响。因此，这些细胞可用于受损CNS的一般修复。免疫磁珠法可从成年大鼠嗅球融合培养物中分离纯化出大量的嗅球内皮细胞。活的内皮细胞可以培养和纯化，在良好的产量从OB解剖至18小时后死亡。纯化的EC可以在-75 ℃下冷冻储存至少6个月，同时保持95%的活力。用荧光细胞接头PKH-26标记的EC既不脱落标记，也不与其他细胞交换标记。移植到成年海马的标记的ECs的迁移进行了检查，从3小时到30天的时间间隔。移植后4天，首次观察到从注射部位的主动迁移，此时EC出现在海马和齿状核细胞层的神经元之间。30天后，一些EC仍然在该位置，但在那个时候，可以观察到的嗅神经胶质细胞的位点作为遥远的和不同的丘脑背侧核，内囊，弓状核，大脑导水管壁和脉络丛。内皮细胞似乎有偏好的齿状门，锥体和颗粒细胞层，脉络丛，血管和推定的肽能位点。
Regenerating nerve fiber sprouts enveloped by olfactory bulb (OB) ensheathing cells (ECs) seem to escape the inhibitory influence of gliotic tissue. Accordingly, these cells may be useful for general repair of injured CNS. Relatively large numbers of ECs could be purified from confluent cultures of adult rat olfactory bulb using immunomagnetic beads. Viable ECs could be cultured and purified in good yield from OB dissected up to 18 h post-mortem. Purified ECs could be stored frozen at -75 degrees C for at least 6 months, while maintaining 95% viability. ECs labelled with the fluorescent cell-linker PKH-26 neither shed the label nor exchanged it with other cells. The migration of labelled ECs transplanted to adult hippocampus was examined at intervals ranging from 3 h to 30 days. Active migration from the injection site was first observed 4 days after transplantation, when ECs appeared intercalated between the neurons of the hippocampal and dentate cell layers. Some ECs remained in that location after 30 days but, at that time, the olfactory glial cells could be observed in loci as distant and diverse as the laterodorsal thalamic nuclei, internal capsule, arcuate nucleus, cerebral aqueduct walls and choroid plexus. ECs seemed to have preferences for the dentate hilus, the pyramidal and granular cell layers, choroid plexus, blood vessels and putative peptidergic loci.