Expression of 5-HT7 receptor mRNA in the hamster brain:: Effect of aging and association with calbindin-D28K expression
Expression of 5-HT7 receptor mRNA in the hamster brain:: Effect of aging and association with calbindin-D28K expression
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DOI：
10.1016/j.brainres.2007.01.044
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发表时间：
2007-04-27
期刊：
影响因子：
2.9
通讯作者：
Franklin, Kathleen M.
中科院分区：
文献类型：
--
作者：
Duncan, Marilyn J.;Franklin, Kathleen M.
衰老影响由S-HT 7受体亚型调节的几个过程，包括昼夜节律，学习和记忆以及抑郁症。以前，我们表明，老化诱导减少仓鼠中缝背核（DRN）在5-HT 7受体结合和昼夜节律相位重置反应8-OH-DPAT显微注射。为了阐明5-HT 7受体衰老减少的机制，我们研究了DRN、DRN传入脑区和调节昼夜节律或记忆的脑区中5-HT 7受体mRNA表达的衰老调制。在从年轻、中年和老年雄性叙利亚仓鼠的脑制备的冠状切片上进行5-HT 7受体mRNA的原位杂交。通过X射线胶片放射自显影的光密度测定法定量5-HT 7受体mRNA表达。结果表明，老化没有显着影响5-HT 7受体mRNA的表达在DRN或大多数其他脑区检查，除了扣带皮层和室旁丘脑核。在视交叉上核，在哺乳动物中的主昼夜节律起搏器的网站，5-HT 7受体mRNA的表达被定位在一个离散的亚区类似的钙结合蛋白亚核先前描述。使用相邻组织切片的第二个实验表明，5-HT 7受体mRNA和钙结合蛋白mRNA集中在SCN的同一区域，以及其他几个脑结构的同一区域。5-HT 7受体和钙结合蛋白mRNA在相同区域内的定位表明它们编码的蛋白质可能相互作用以调节诸如昼夜节律计时的过程。(c)2007 Elsevier B. V.保留所有权利。
Aging affects several processes modulated by the S-HT7 receptor subtype, including circadian rhythms, learning and memory, and depression. Previously, we showed that aging induces a decrease in the hamster dorsal raphe (DRN) in both 5-HT7 receptor binding and circadian phase resetting responses to 8-OH-DPAT microinjection. To elucidate the mechanisms underlying the aging decrease in 5-HT7 receptors, we investigated aging modulation of 5-HT7 receptor mRNA expression in the DRN, brain regions afferent to the DRN, and brain regions regulating circadian rhythms or memory. in situ hybridization for 5-HT7 receptor mRNA was performed on coronal sections prepared from the brains of young, middle-aged, and old male Syrian hamsters. 5-HT7 receptor mRNA expression was quantified by densitometry of X-ray film autoradiograms. The results showed that aging did not significantly affect 5-HT7 receptor mRNA expression in the DRN or most other brain regions examined, with the exception of the cingulate cortex and paraventricular thalamic nucleus. Within the suprachiasmatic nucleus, the site of the master circadian pacemaker in mammals, 5-HT7 receptor mRNA expression was localized in a discrete subregion resembling the calbindin subnucleus previously described. A second experiment using adjacent tissue sections showed that 5-HT7 receptor mRNA and calbindin mRNAs were concentrated in the same region of the SCN, and as well as in the same region of several other brain structures. The localization of 5-HT7 receptors and calbindin mRNAs within the same regions suggests that the proteins they encode may interact to modulate processes such as circadian timekeeping. (c) 2007 Elsevier B.V. All rights reserved.