Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.
Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.
复制标题
DOI：
10.1021/ac00002a005
复制
发表时间：
1991-01
影响因子：
7.4
通讯作者：
M. Moseley;L. Deterding;K. Tomer;J. Jorgenson
中科院分区：
文献类型：
--
作者：
M. Moseley;L. Deterding;K. Tomer;J. Jorgenson
采用同轴连续流快原子轰击接口，通过毛细管区带电泳/质谱（CZE/MS）分析了生物活性肽的混合物。从低飞摩尔水平的肽获得高分离效率（高达410，000理论塔板）。碱性肽的分析是通过使用氨丙基甲硅烷基化的CZE柱，以尽量减少由于吸附效应而导致的区带展宽来完成的。CZE/MS/MS数据在电泳真实的时间中从飞摩尔水平的肽获得。
Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.