Screening of non starch polysaccharide degrading enzymes and enzyme combinations for their ability to degrade non starch polysaccharides of broiler diet by two stage in vitro digestion assay
Screening of non starch polysaccharide degrading enzymes and enzyme combinations for their ability to degrade non starch polysaccharides of broiler diet by two stage in vitro digestion assay
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发表时间：
2013
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通讯作者：
J. Narasimha;D. Nagalakshmi;Y. Reddy;S. T. V. Rao
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作者：
J. Narasimha;D. Nagalakshmi;Y. Reddy;S. T. V. Rao
本试验旨在寻找一种适宜的NSP酶组合，以提高肉仔鸡玉米和豆粕型饲粮的消化率。通过两期体外NSP消化率试验，确定木聚糖酶、纤维素酶和β- d -葡聚糖酶在玉米-豆粕型肉仔鸡饲粮中添加或不添加蛋白酶的合适NSP酶组合。通过测定总糖的释放量来测定NSP的消化程度。通过肉仔鸡体外消化率试验筛选不同浓度的非淀粉多糖酶，即木聚糖酶（0 ~ 60000 IU/kg）、纤维素酶（0 ~ 6400 IU/kg）、β- d -葡聚糖酶（0 ~ 6400 IU/kg）和蛋白酶（0 ~ 64000 IU/kg）。从中选择3种最佳的NSP酶浓度，为每种酶配制27种（3x3x3）酶组合。在蛋白酶的情况下，选择了五个浓度。5000、7500和10000木聚糖酶IU/kg浓度显著提高了肉仔鸡饲粮的体外糖释放量（P<0.01）；50、100、400纤维素酶IU/kg；β- d -葡聚糖酶100、200、400 IU/kg和蛋白酶500、1000、2000、4000、8000 IU/kg饲粮。在27种木聚糖酶、纤维素酶和β- d -葡聚糖酶组合中，选择木聚糖酶7500、纤维素酶100和β- d -葡聚糖酶100 IU/kg，加或不加2000 IU蛋白酶/kg的组合。由此可见，模拟家禽肠道条件的NSP体外消化率试验可作为一种简单有效的技术，为不同饲料成分配制肉鸡的饲粮进化出适合的NSP酶组合和酶的最有效浓度，并获得最佳有效剂量水平。
The investigation was undertaken to evolve a suitable NSP enzyme combination with potential to improve the digestibility of corn and soybean meal based diets for broilers. Two stage in vitro NSP digestibility assay was carried to arrive at a suitable NSP enzyme combination comprising of xylanase, cellulase and β-D-glucanase with or without protease for corn-soybean meal based broiler diets. The degree of NSP digestibility was assayed by measuring the amount of total sugars released. Various concentrations of Non-starch Polysaccharide enzymes viz., xylanase (0-60000 IU/kg), cellulase (0–6400 IU/kg), β-D-glucanase (0–6400 IU/kg) and protease (0-64000 IU/kg) were screened by in vitro digestibility assay for broilers. From that three best concentrations of NSP enzymes were selected for each enzyme to formulate twenty seven (3x3x3) enzyme combinations. In case of protease five concentrations were selected. The in vitro sugars release for broiler diet was significantly higher (P<0.01) for concentrations of 5000, 7500 and 10000 xylanase IU/kg; 50, 100 and 400 cellulase IU/kg; 100, 200 and 400 β-D-glucanase IU/kg and 500, 1000, 2000, 4000 and 8000 protease IU/kg diet. Among the twenty seven enzyme combinations of xylanase, cellulase and β-D-glucanase, the combination comprising of xylanase-7500, cellulase-100 and β-D-glucanase-100 IU/kg and with or without 2000 IU protease/kg was selected for broiler diet. It can be concluded that In vitro NSP digestibility assay, simulating the gut conditions of poultry could be used as a simple and effective technique to evolve suitable NSP enzyme combination with most effective concentration of enzymes specifically for diets of broilers formulated with various feed ingredients and also to arrive at best effective dosage level.