The Protein Encoded by NCgl1221 in Corynebacterium glutamicum Functions as a Mechanosensitive Channel
The Protein Encoded by NCgl1221 in Corynebacterium glutamicum Functions as a Mechanosensitive Channel
复制标题
DOI：
10.1271/bbb.100636
复制
发表时间：
2010-12-01
影响因子：
1.6
通讯作者：
Kawasaki, Hisashi
中科院分区：
文献类型：
--
作者：
Hashimoto, Ken-ichi;Nakamura, Koji;Kawasaki, Hisashi
谷氨酸棒杆菌NCgl1221编码的蛋白质的功能进行了分析，使用枯草芽孢杆菌作为宿主，因为已经建立了制备电生理学研究所需的巨大provacuole的方法。NCgl1221在mscL和ykuT（两者都编码机械敏感性通道）缺陷的菌株中的表达导致在渗透压下休克时比对照高8.9倍的细胞存活率。电生理研究表明，从该菌株制备的巨大的provacuole，表达NCgl1221，表现出显着更高的压力依赖性电导比对照。这些发现表明NCgl1221编码的蛋白质作为机械敏感通道发挥功能。
The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressure-dependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.