CHARACTERIZATION OF ENVELOPE AND CORE STRUCTURAL GENE-PRODUCTS OF HTLV-III WITH SERA FROM AIDS PATIENTS
CHARACTERIZATION OF ENVELOPE AND CORE STRUCTURAL GENE-PRODUCTS OF HTLV-III WITH SERA FROM AIDS PATIENTS
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DOI：
10.1126/science.2984774
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
FISCHINGER, PJ
中科院分区：
文献类型：
--
作者：
ROBEY, WG;SAFAI, B;FISCHINGER, PJ
用免疫亲和层析、免疫沉淀和二维寡肽图谱分析方法，鉴定了人T细胞白血病病毒Ⅲ型的包膜（env）和结构（gag）基因产物。env基因指定一种分子量为160，000的糖基化多肽（gp160），将其加工成gp120和更小的基因产物。gag基因指定70，000和55，000 MW的两种多肽（p70和p55），这两种多肽都含有成熟病毒粒子的主要结构蛋白p24。本研究中的技术可用于确定不同病毒分离株之间env基因产物的变异程度，并可鉴定导致免疫保护状态的体液免疫应答的性质和模式。
The envelope (env) and structural (gag) gene products of human T-cell leukemia (lymphotropic) virus type III were identified by immunoaffinity chromatography, immunoprecipitation and 2-dimensional oligopeptide mapping methods. The env gene specifies a glycosylated polypeptide with a MW of 160,000 (gp160) that is processed to gp120 and smaller gene products. The gag gene specifies 2 polypeptides of 70,000 and 55,000 MW (p70 and p55), both of which contain p24, the major structural protein of the mature virion. The techniques in this study can be used to define the extent of variability of the env gene product among different virus isolates and may identify the nature and patterns of the humoral immune response that lead to an immunologically protected state.