YB-1 overexpression promotes a TGF-β1-induced epithelial-mesenchymal transition via Akt activation
YB-1 overexpression promotes a TGF-β1-induced epithelial-mesenchymal transition via Akt activation
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DOI：
10.1016/j.bbrc.2015.01.114
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发表时间：
2015-03-06
影响因子：
3.1
通讯作者：
Jang, Ho Hee
中科院分区：
文献类型：
--
作者：
Ha, Bin;Lee, Eun Byul;Jang, Ho Hee
Y盒结合蛋白-1（YB-1）是转录/翻译调节蛋白，其表达与癌症侵袭性相关。在本研究中，我们探讨了YB-1在转化生长因子-β 1（TGF-β 1）诱导的肺腺癌细胞上皮间质转化（EMT）中的调节作用。下调YB-1表达可增加E-cadherin启动子活性，上调YB-1表达可降低E-cadherin启动子活性，提示YB-1水平可能与EMT相关。TGF-β 1诱导YB-1表达，TGF-β 1将胞质YB-1转运到细胞核中。YB-1过表达促进TGF-β 1诱导的上皮标志物下调、间充质标志物上调和细胞迁移。此外，YB-1过表达通过TGF-β 1诱导的Akt激活增强E-钙粘蛋白转录抑制因子的表达。我们的发现为YB-1在TGF-β 1信号通路中所起的作用提供了新的见解。(C)2015爱思唯尔公司All rights reserved.
The Y-box binding protein-1 (YB-1) is a transcription/translation regulatory protein, and the expression thereof is associated with cancer aggressiveness. In the present study, we explored the regulatory effects of YB-1 during the transforming growth factor-beta 1 (TGF-beta 1)-induced epithelial-to-mesenchymal transition (EMT) in lung adenocarcinoma cells. Downregulation of YB-1 increased E-cadherin promoter activity, and upregulation of YB-1 decreased promoter activity, suggesting that the YB-1 level may be correlated with the EMT. TGF-beta 1 induced YB-1 expression, and TGF-beta 1 translocated cytosolic YB-1 into the nucleus. YB-1 overexpression promoted TGF-beta 1-induced downregulation of epithelial markers, upregulation of mesenchymal markers, and cell migration. Moreover, YB-1 overexpression enhanced the expression of E-cadherin transcriptional repressors via TGF-beta 1-induced Akt activation. Our findings afford new insights into the role played by YB-1 in the TGF-beta 1 signaling pathway. (C) 2015 Elsevier Inc. All rights reserved.