Endobronchial secretion of interleukin-13 following local allergen challenge in atopic asthma: Relationship to interleukin-4 and eosinophil counts
Endobronchial secretion of interleukin-13 following local allergen challenge in atopic asthma: Relationship to interleukin-4 and eosinophil counts
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DOI：
10.1183/09031936.96.09050899
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发表时间：
1996-05-01
影响因子：
24.3
通讯作者：
Luttmann, W
中科院分区：
文献类型：
--
作者：
Kroegel, C;Julius, P;Luttmann, W
我们研究了10例轻度变应性哮喘患者气道中白细胞介素(IL)-13的分泌情况，并将其与支气管肺泡灌洗（BAL）获得的IL-4水平和嗜酸性粒细胞数量进行了比较。内镜下将适当的过敏原或生理盐水注入不同气道段，在过敏原或假攻毒后10 min和18 h灌洗，采用双夹心酶联免疫吸附试验（ELISA）检测未浓缩BAL液中IL-4和IL-13的含量。支气管内致敏原刺激在致敏原暴露段18 h后诱导嗜酸性粒细胞数量显著增加。暴露于过敏原10分钟后，可以检测到低水平的IL-4和IL-13，而在局部暴露于过敏原18小时后，这两种细胞因子的浓度显著升高。与IL-4相比，IL-13的浓度与嗜酸性粒细胞数量在过敏原激发后18 h出现了强烈的相关性。结果提示，轻度哮喘患者在哮喘反应后期，白细胞介素-13的分泌较为活跃。鉴于其对嗜酸性粒细胞和其他细胞类型的作用，我们认为除了白细胞介素-4外，白细胞介素-13可能在嗜酸性粒细胞相关炎症（如支气管哮喘）的发病机制中发挥重要作用。
We investigated the secretion of interleukin (IL)-13 into the airways in 10 mild allergic asthmatics by employing local allergen challenge, and compared the data both to IL-4 levels and eosinophil numbers obtained by bronchoalveolar lavage (BAL).Appropriate allergen or saline were endoscopically instilled into different airway segments, which were lavaged 10 min and 18 h after allergen or sham challenge, IL-4 and IL-13 were measured in unconcentrated BAL fluid using a double sandwich enzymes-linked immunosorbent assay (ELISA).Endobronchial allergen challenge induced a highly significant increase in the numbers of eosinophils after 18 h in the allergen exposed segment. Ten minutes following allergen exposure, low levels of IL-4 and IL-13 could be detected, whilst concentrations of both cytokines were significantly raised 18 h following local allergen exposure. In contrast to IL-4, the concentration of IL-13 strongly correlated with the eosinophil numbers found 18 h post-allergen challenge.The results suggest that interleukin-13 is actively secreted during the late asthmatic response in mild asthmatic subjects. In view of its action on eosinophils and other cell types, we conclude that interleukin-13, in addition to interleukin-4, may play an important role in the pathogenesis of eosinophil-related inflammation, such as bronchial asthma.