Mapping wound-response genes involved in post-harvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz)
Mapping wound-response genes involved in post-harvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz)
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DOI：
10.1023/a:1020695719706
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发表时间：
2002-11-01
期刊：
影响因子：
1.9
通讯作者：
Tohme, J
中科院分区：
文献类型：
--
作者：
Cortés, DF;Reilly, K;Tohme, J
对木薯采后生理劣变过程中表达的损伤反应基因如苯丙氨酸解氨酶、β-1.3葡聚糖酶、富含羟脯氨酸的糖蛋白、过氧化氢酶、1-氨基环丙烷1-羧酸、半胱氨酸蛋白酶抑制剂、天冬氨酸蛋白酶、丝氨酸/苏氨酸蛋白激酶和过氧化物酶的部分cDNA、已在木薯分子遗传框架图上得到鉴定。此外，分子标记连锁的推定数量性状基因座（QTL）影响木薯PPD使用F（1）作图群体来自优良亲本系（TMS 30572 × cm 2177-2）。利用240个RFLP标记、100个RAPD标记、85个微卫星标记和5个同工酶标记对144个F1单株进行了QTL定位。基于< 0.005的概率值鉴定了与PPD的推定QTL具有显著关联的一组10个分子标记，以最小化假阳性的检测。基于单标记回归分析，在母本框架图的G、P、L、U和X连锁群上发现了8个QTL，它们解释了PPD表型变异的5-12%。在雄性框架图中，位于连锁群C和L上的两个QTL分别解释了13%和11%的变异。因此，本研究确定了木薯的主要基因组区域相关的生理收获后的恶化，从而提供了工具，用于识别基因（S）控制这一性状。
The genome locations of the wound-response genes that were expressed during the post-harvest physiological deterioration (PPD) of cassava, such as phenylalanine ammonia lyase, beta-1.3 glucanase, hydroxyproline rich glycoprotein, catalase, 1-aminocyclopropane 1-carboxylate, cysteine protease inhibitor, aspartic protease, a partial cDNA for serine/threonine protein kinase and peroxidase, have been identified on the framework molecular genetic map of cassava. Also, molecular markers linked to putative quantitative trait loci (QTLs) influencing PPD of cassava were mapped using an F(1)mapping population derived from elite parental lines (TMS 30572 x cm 2177-2). A molecular linkage map previously constructed based on the segregation of 240 RFLP, 100 RAPD, 85 microsatellite and five isoenzyme markers on 144 F-1 individuals was used for the QTL mapping. A set of 10 molecular markers with a significant association with putative QTLs for PPD were identified based on probability values < 0.005 in order to minimize the detection of false positives. Based on single-marker regression, eight putative QTLs located on the linkage groups G, P, L, U, and X of the female-derived framework map were found to explain between 5-12% of the phenotypic variance of the PPD. In the male-derived framework map, two putative QTLs on linkage groups C and L explained 13% and 11% of this variance, respectively. This study thus identified the major genome regions of cassava related to physiological post-harvest deterioration, thereby providing tools for the identification of gene(s) controlling this trait.