Local secretion of complement C3 in the exocrine pancreas: Ductal epithelial cells as a possible biosynthetic site
Local secretion of complement C3 in the exocrine pancreas: Ductal epithelial cells as a possible biosynthetic site
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DOI：
10.1053/gast.1996.v110.pm8964419
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发表时间：
1996-06-01
期刊：
影响因子：
29.4
通讯作者：
Bamba, T
中科院分区：
文献类型：
--
作者：
Andoh, A;Fujiyama, Y;Bamba, T
背景与目的：补体系统参与多种组织的局部免疫系统，本研究探讨了补体C3在胰液中的局部分泌，并试图确定其可能的生物合成位点。方法：采用免疫印迹法分析人胰液中C3蛋白。用聚合酶链反应和/或Northern印迹法分析了几种胰腺癌细胞株中C3信使RNA （mRNA）的表达，用代谢标记和免疫沉淀实验评价了这些胰腺癌细胞分泌C3的情况。在5个人胰液样本中，检测到C3是由α和β链组成的分子，C3 mRNA在导管细胞癌细胞系（PANC-1和MIA PaCa-2）中表达，而在腺泡细胞系（HPC-YO和AR-42J）中未表达，白细胞介素1 β和肿瘤坏死因子α在蛋白质和mRNA水平上增强了这些细胞的C3生成。结论：(1)补体C3分泌到胰腺外分泌液中；(2)导管上皮细胞可能是C3的生物合成部位；(3)促炎因子、白细胞介素1 β和肿瘤坏死因子α是胰腺局部C3生成的有效刺激物。
Background & Aims: The complement system participates in the local immune system in various tissues, In this study, we investigated the local secretion of complement C3 into the pancreatic fluid and attempted to determine a possible biosynthetic site, Methods: C3 protein in human pancreatic fluid was analyzed by immunoblotting. The C3 messenger RNA (mRNA) expression in several pancreatic carcinoma cell lines was analyzed by the polymerase chain reaction and/or Northern blotting, The secretion of C3 by these pancreatic carcinoma cells was assessed by metabolic labeling and immunoprecipitation experiments, Results: In five samples of human pancreatic fluid, C3 was detected as a molecule composed of alpha and beta chains, C3 mRNA expression was observed in the ductal cell carcinoma lines (PANC-1 and MIA PaCa-2) but not in the acinar cell line (HPC-YO and AR-42J), C3 production in these cells was enhanced by interleukin 1 beta and tumor necrosis factor alpha at both the protein and the mRNA levels. Conclusions: (1) Complement C3 is secreted into the exocrine fluids of the pancreas, (2) Ductal epithelial cells are possible biosynthetic sites for C3, (3) The proinflammatory cytokines, interleukin 1 beta and tumor necrosis factor alpha are effective stimulators of local C3 production in the pancreas.