Binding of rabbit hemorrhagic disease virus to antigens of the ABH histo-blood group family
Binding of rabbit hemorrhagic disease virus to antigens of the ABH histo-blood group family
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DOI：
10.1128/jvi.74.24.11950-11954.2000
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Le Pendu, J
中科院分区：
文献类型：
--
作者：
Ruvoën-Clouet, N;Ganière, JP;Le Pendu, J
兔出血性疾病病毒凝集人红细胞和附着于兔上呼吸道和消化道上皮细胞的能力取决于ABH血型抗原的存在。事实上，分泌个体的唾液能抑制凝集，而非分泌个体的唾液则不能，后者缺乏H抗原。此外，罕见的孟买型红细胞，完全缺乏ABH抗原，不凝集。从感染兔肝脏提取的天然病毒颗粒，以及从重组病毒衣壳蛋白中提取的病毒样颗粒，特异性结合合成的A型和H型2型血型低聚糖。这两种类型的颗粒都能附着在成年兔上呼吸道和消化道的上皮细胞上。这种结合与抗H 2型血型试剂的结合相似，并被H 2型特异性凝集素UEA-I和聚丙烯酰胺偶联的H 2型三糖抑制。幼兔组织中几乎没有A和H 2型抗原，病毒颗粒只能在这些组织上得到非常弱的结合。
The ability of rabbit hemorrhagic disease virus to agglutinate human erythrocytes and to attach to rabbit epithelial cells of the upper respiratory and digestive tracts was shown to depend on the presence of ABH blood group antigens. Indeed, agglutination was inhibited by saliva from secretor individuals but not from nonsecretors, the latter being devoid of H antigen. In addition, erythrocytes of the rare Bombay phenotype, which completely lack ABH antigens, were not agglutinated. Native viral particles from extracts of infected rabbit liver as well as virus-like particles from the recombinant virus capsid protein specifically bound to synthetic A and H type 2 blood group oligosaccharides. Both types of particles could attach to adult rabbit epithelial cells of the upper respiratory and digestive tracts. This binding paralleled that of anti-H type 2 blood group reagents and was inhibited by the H type 2-specific lectin UEA-I and polyacrylamide-conjugated H type 2 trisaccharide. Young rabbit tissues were almost devoid of A and H type 2 antigens, and only very weak binding of virus particles could be obtained on these tissues.