Shinella granuli gen nov, sp nov, and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp nov
Shinella granuli gen nov, sp nov, and proposal of the reclassification of Zoogloea ramigera ATCC 19623 as Shinella zoogloeoides sp nov
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DOI：
10.1099/ijs.0.63942-0
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发表时间：
2006-02-01
影响因子：
2.8
通讯作者：
Lee, ST
中科院分区：
文献类型：
--
作者：
An, DS;Im, WT;Lee, ST
对从升流式厌氧污泥床反应器中分离出的一株新型细菌菌株Ch06(T)的分类地位进行了确定。菌株Ch06(T)为革兰氏阴性、好氧、可运动，氧化酶和过氧化氢酶呈阳性。16S rRNA基因序列的比较分析表明，菌株Ch06(T)明显属于α-变形菌纲，且与动胶菌属（Zoogloea）的动胶菌（Zoogloea ramigera）ATCC 19623和双型枝原体属（Mycoplana）的双型枝原体（Mycoplana dimorpha）IAM 13154(T)亲缘关系最为密切（序列相似性分别为97.9%和96.3%）。主要的呼吸醌为Q - 10，主要的脂肪酸为C - 16:0、3 - OH C - 16:0、C - 18:0、C - 19:0环ω8c以及总特征7（C(18:1)ω7c/ω9t/ω12t，C(18:1)ω7c/ω12t）。基于表型、化学分类和系统发育特征，该新型菌株被归入一个新属，申氏菌属（Shinella gen. nov.），为颗粒申氏菌（Shinella granuli gen. nov.，sp. nov.）（模式菌株Ch06(T)=KCTC 12237(T)=JCM 13254(T)）。建议将动胶菌属的动胶菌（Zoogloea ramigera）ATCC 19623重新分类到新属申氏菌属中，作为类动胶申氏菌（Shinella zoogloeoides sp. nov.）（模式菌株ATCC 19623(T)=IAM 12669(T)=I - 16 - M - T）
The taxonomic position of a novel bacterial strain, Ch06(T), isolated from an upflow anaerobic sludge blanket reactor was determined. Strain Ch06T was Gram-negative, aerobic, motile and oxidase- and catalase-positive. A comparative 16S rRNA gene sequence analysis showed a clear affiliation of strain Ch06(T) to the Alphaproteobacteria and it was most closely related to Zoogloea ramigera ATCC 19623 and Mycoplana dimorpha IAM 13154(T) (97.9 and 96.3% sequence similarity, respectively). The major respiratory quinone was Q-10 and the predominant fatty acids were C-16:0, 3-OH C-16:0, C-18:0, C-19:0 cyclo omega 8c and summed feature 7 (C(18:1)omega 7c/omega 9t/omega 12t, C(18:1)omega 7c/omega 12t). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the novel isolate was assigned to a new genus, Shinella gen. nov., as Shinella granuli gen. nov., sp. nov. (type strain Ch06(T) =KCTC 12237(T) = JCM 13254(T)). It is proposed that Zoogloea ramigera ATCC 19623 is reclassified into the novel genus Shinella as Shinella zoogloeoides sp. nov. (type strain ATCC 19623(T) = IAM 12669(T) = I-16-M-T).