Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge
Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge
复制标题
用编码艾美耳球虫 3-1E 和鸡 IL-15 的 DNA 疫苗对鸡进行疫苗接种，可提供针对同源攻击的保护
DOI：
10.1016/j.exppara.2010.07.015
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Ren, Xiaofeng
中科院分区：
文献类型：
--
作者：
Ma, Dexing;Ma, Chunli;Ren, Xiaofeng
将堆型艾美耳球虫3- 1 E抗原基因和成熟鸡白细胞介素15（mChIL-15）基因以不同的形式克隆到真核表达载体pcDNA3.1（+）中，制备了DNA疫苗pcDNA3.1-3- 1 E和共表达的pcDNA3.1-3- 1 E-linker-mChIL-15。acervulina 3- 1 E基因和mChIL-15基因。采用间接荧光抗体技术和免疫组织化学方法检测目的基因的体外表达。两种DNA疫苗通过腿部肌肉注射施用。通过动物攻毒试验评价疫苗的免疫保护效果。结果表明，成功构建了DNA疫苗，并能在体外检测到目的基因的表达。动物实验结果表明，两种DNA疫苗均能部分保护鸡抵抗同种攻击。与pcDNA3.1-3- 1 E相比，pcDNA3.1-3- 1 E-linker-mChIL-15嵌合DNA疫苗能显著提高卵囊减少率，降低十二指肠平均病变评分，提高体重增长，提高抗球虫指数（ACI）。这些结果表明，ChIL-15能增强3- 1 E DNA疫苗的免疫原性，细胞因子与优化的艾美耳球虫表面抗原共表达有望成为一种提高DNA疫苗免疫原性的方法。(C)2010年爱思唯尔公司All rights reserved.
Eimeria acervulina 3-1E antigen gene and mature chicken interleukin 15 (mChIL-15) gene were cloned into expression vector pcDNA3.1(+) in different forms, produced DNA vaccine pcDNA3.1-3-1E, and pcDNA3.1-3-1E-linker-mChIL-15 co-expressing E. acervulina 3-1E gene and mChIL-15 gene, respectively. The expression of objective gene in vitro was detected by indirect fluorescent antibody technique and immunohistochemistry. The two DNA vaccines were administered by intramuscular leg injection. An animal challenge experiment was carried out to evaluate the immune protective efficacy of the vaccines. The results indicated that DNA vaccines were successfully constructed and the expression of objective gene could be detected in vitro. The animal experimental results showed that both DNA vaccines could provide partial protection against homologous challenge in chickens. The chimeric DNA vaccine, pcDNA3.1-3-1E-linker-mChIL-15, could significantly increase oocyst decrease ratio, reduce the average lesion score in the duodenum, improve body weight gain, and increase anti-coccidial index (ACI) compared to the DNA vaccine pcDNA3.1-3-1E. Taken together, these results demonstrate ChIL-15 enhance the immunogenicity of 3-1E DNA vaccine, and co-expression of cytokine and optimized surface antigen of Eimeria may be a promising method to enhance immunogenicity of DNA vaccines in poultry. (C) 2010 Elsevier Inc. All rights reserved.