Evidence for sub-families of actin genes in Dictyostelium as determined by comparisons of 3' end sequences.
Evidence for sub-families of actin genes in Dictyostelium as determined by comparisons of 3' end sequences.
复制标题
通过比较 3 末端序列确定网柄菌中肌动蛋白基因亚家族的证据。
DOI：
10.1016/0022-2836(81)90425-3
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发表时间：
1981
影响因子：
5.6
通讯作者：
Firtel,RA
中科院分区：
文献类型：
--
作者：
McKeown,M;Firtel,RA
我们已经测定了五个肌动蛋白基因组克隆和三个肌动蛋白互补DNA克隆的3‘端序列。序列比较表明，蛋白质编码区高度保守，而与3‘非翻译区对应的区域存在差异。另外的分析表明，肌动蛋白基因之间的3‘非翻译区存在同源性。用标记的3‘端进行的Southern DNA印迹杂交研究表明，在3’非翻译区中存在与肌动蛋白基因相关的亚家族。在信使RNA编码区以外的序列中没有发现同源性。对序列数据的分析表明，肌动蛋白信使RNA的~1.25×103和~1.35×103碱基之间的长度差异在3‘非翻译区。
We have sequenced the 3′ end of five actin genomic clones and three actin complementary DNA clones fromDictyostelium. Comparison of the sequences shows that the protein coding regions are highly conserved, while the region corresponding to the 3′ untranslated regions are divergent. Additional analysis indicates regions of homology in the 3′ untranslated region between sets of actin genes. Southern DNA blot hybridization studies using labeled 3′ ends suggest that there are sub-families of actin genes that are related within the 3′ untranslated regions. No homology is found in the sequences outside the messenger RNA encoding regions. Analysis of the sequence data has shown that the difference in length between the ~1.25 × 103and ~1.35 × 103base actin messenger RNAs is in the lengths of the 3′ untranslated region.